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Nanolight/Prolume Purple/3691 ( 2 x500ug)/1 mg

Prolume Purpleormethoxye-Coelenterazine(Me-O-e-CTZ)isanewsyntheticanalogueof e-Coelenterazinewithanadditional methoxygroupquenchingtheemissionmaximumfrom 475nmto405nm. Nanolight™Technologies developedProlumePurple. (USPatent2013/057660)

Prolume Purple exhibitsanupto13-foldhigherluminescencethanCTZ400a(“deepblueC”).
Pleaseseeour
"Howtouse"forfurtherinformation. 

 

CompoundID:ProlumePurple(PP-CTZ)
alternativename:methoxye-Coelenterazine(me-O-eCTZ)
MolecularFormula:C29H25N3O3
MW:463.53
Structure:
Application:emitsaround405nmwithRLuc,RLucII,RLuc3,RLuc8andGreenRenillaLuc
Solvent:WerecommendNanoFuelSolvent.LowsolubilityinEthanol.
comparisontoCTZ400a: 
EmissionspecwithRenillaLuciferases: 
References:

BioluminescenceAssistedSwitchingandFluorescenceImaging(BASFI)
LuyuanZhang,FangXu,ZhixingChen,XinxinZhuandWeiMin. 
J.Phys.Chem.Lett.,2013,4,pp3897-3902

ProlumePurple(Cat.#369)wasenablingtheseresearchestofollowproteinmovementwithinasinglecellinreal-timeforupto20minusingasophisticatedBRETsystem:

MonitoringGprotein-coupledreceptorandbeta-arrestintraffickinginlivecellsusingenhancedbystanderBRET

YoonNamkung,ChristianLeGouill,ViktoriaLukashova,HiroyukiKobayashi,MireilleHougue,EtienneKhoury,MideumSong,MicharlBouvier,StephaneA.Laporte

 

HowtouseProlumePurple

CertificateofAnalysis  HPLC  MSDS  NMR

Theluciferinisshippedasfiltered,lyophilized,batchcontrolledsubstrate in250ugand500ugaliquots.
1mg=2x500ug. 10mgsispackedasregularpowderinonetube.

Theadvantagesoflyophilizedluciferinsare:

  • pre-aliquotedinsmallamounts,freshsubstrateforeverysetofexperiments
  • longshelf-life(packedunderArgon)

 


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