Nanolight/Coelenterazine 400a/34010 (2 x5mgs)/10 mgs

CanbeoxidizedbyRenillaluciferases(Rluc)butnotGaussialuciferase.Itisusedforreal-timedetectionofcalciumions,genereporterassaysandafavoredsubstrateforBRET.Thisderivatehasanemissionmaximumaround400nmandthereforeminimalinterferencewiththeemissionoftheGFPacceptor.

CompoundID:	Coelenterazine400a,1-bisdeoxycoelenterazine,di-dehydrocoelenterazine,DeepBlueCTM
CAS#	70217-82-2
IUPACName:	2,8-DIBENZYL-6-PHENYL-IMIDAZO[1,2A]PYRAZIN-3-(7H)-1; BISDEOXYCOELENTERAZINE
MolecularFormula:	C26H21N3O
MW:	391.5
Structure:

HowtoUseCoelenterazine400a

CertificateofAnalysis    HPLC     MSDS    NMR

Theluciferinisshippedasfiltered,lyophilized,batchcontrolledsubstrate
in500µgaliquots(1mg=2x500µg)andin5mgsand10mgs(2x5mgs)aliquots. 

Theadvantagesoflyophilizedluciferinsare:

• pre-aliquotedinsmallamounts,freshsubstrateforeverysetofexperiments
• longshelf-life(packedunderArgon)
• fastertodissolveduetoitsfinecrystalstructure
• consistentqualitybetweenaliquotes

Shipping:viaFedExorUPSatRT,overnightor2nddelivery.Storeat-80°C.Protectfromlight.