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TCO(tetrazine–trans-cyclooctene)ligationisoneofthefastestreactionsusedforbioconjugation.Itisaninverseelectrondemand[4+2]cycloadditionthattakesplacebetweentetrazineandtrans-cycloocteneorotherstrainedolefin.
Sulfo-Cyanine5tetrazineisafluorophorederivativebearingatetrazinegroupfortheTCO-ligationbasedlabeling.
ThisreagentpossessesgoodaqueoussolubilityandstABIlityinBIOLOGicalenvironments.
| Appearance: | darkbluesolid |
| Molecularweight: | 864.09 |
| Molecularformula: | C42H46KN7O7S2 |
| Solubility: | goodinwater,DMF,DMSO |
| Qualitycontrol: | NMR1H,HPLC-MS(95%) |
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
| Excitationmaximum,nm: | 646 |
| ε,L⋅mol−1⋅cm−1: | 271000 |
| Emissionmaximum,nm: | 662 |
| Fluorescencequantumyield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |