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Thiolmono-reactiveCyanine3dye.ThisreagentcanbeusedtoattachCyanine3fluorophore(ananalogofCy3®)toproteinsandpeptidescontainingcysteineresidues,aswellastootherthiolatedmolecules(suchasthiol-containingoligonucleotides).
CystinesshouldbereducedwithTCEP(tris-carboxyethylphosphine)orotherappropriatereductantpriortothelabeling.
LabelingwithCyanine3maleimideisselective,andefficient.
Werecommendusingwater-solubleSulfo-Cyanine3maleimideforthelabelingofantibodiesandothersensitiveproteins.
| Appearance: | redpowder |
| Molecularweight: | 615.20 |
| Molecularformula: | C36H43ClN4O3 |
| Solubility: | solubleinorganicsolvents(DMF,DMSO,dichloromethane),insolubleinwater |
| Qualitycontrol: | NMR1H,HPLC-MS(95%) |
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
| Excitationmaximum,nm: | 555 |
| ε,L⋅mol−1⋅cm−1: | 150000 |
| Emissionmaximum,nm: | 570 |
| Fluorescencequantumyield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |