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AminereactiveCyanine7,nearinfraredfluorescentdye,animprovedanalogofCy7®.
NIRfluorophorescanbeusedtotakeadvantageofnearinfraredwindowofbiologicaltissues-increasedtransparencyoftissuesinthisspectralregionallowstocarryoutinvivoimaging.
ThisreagentcanbeutilizedtoproduceCyanine7-labeledbiomoleculesforsubsequentuseinvariousinvivoresearch,anddrugdesignrelatedexperiments.
ThestructureofCyanine7featuresrigidizeddesignofcentralpolymethynechain.Thismolecularreinforcementallowstoincreasequantumyieldby20%comparedwithparentstructure,increasingfluorescencebrightness.
Thisreagentrequiresorganicco-solventforthelabeling(pleaseseeRecommendedProtocolssectionbelow).Water-solubleCyanine7NHSesterisalsoavailable,andrecommendedforproteinNIRlabeling.
| Appearance: | greenpowder |
| Molecularweight: | 682.29 |
| Molecularformula: | C41H48ClN3O4 |
| Solubility: | solubleinorganicsolvents(DMSO,DMF,dichloromethane),lowsolubilityinwater |
| Qualitycontrol: | NMR1H,HPLC-MS(95%) |
| Storageconditions: | Storage:12monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
| Excitationmaximum,nm: | 750 |
| ε,L⋅mol−1⋅cm−1: | 199000 |
| Emissionmaximum,nm: | 773 |
| Fluorescencequantumyield: | 0.3 |
| CF260: | 0.022 |
| CF280: | 0.029 |