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Tetrazine-trans-cycloocteneligationisamongthefastestbioconjugationreactionsknowntodate.
Thiswatersolublesulfo-Cyanine3derivativecontainsmethyltetrazinemoietyforthecouplingwithtrans-cyclooctenes.MethyltetrazinespossessoptimalstabilityatphysiologicalpH,whilemaintainingextremelyhighreactivitytowardscyclooctenes.
Thefluorophoreisbright,photostable,andisalsoverywellvisiblewithnakedeye.
| Appearance: | redpowder |
| Molecularweight: | 838.05 |
| Molecularformula: | C40H44KN7O7S2 |
| IUPACname: | Potassium(E)-2-[(E)-3-{3,3-Dimethyl-1-[6-({[p-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl]methyl}amino)-6-oxohexyl]-5-(oxysulfonyl)-3H-indol-2-yl}-2-propenylidene]-1-methyl-3,3-dimethyl-5-indolinesulfonate |
| Solubility: | goodinwater,DMF,DMSO |
| Qualitycontrol: | NMR1H,HPLC-MS(95%) |
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
| Excitationmaximum,nm: | 548 |
| ε,L⋅mol−1⋅cm−1: | 162000 |
| Emissionmaximum,nm: | 563 |
| Fluorescencequantumyield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |