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Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO, and in solid form.
This azide is soluble in organic solvents (e.g. DMSO, DMF), therefore the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.
Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores which is compatible with various instruments. Cyanine5 can also be used as a replacement for Alexa Fluor® 647, and DyLight® 649.
| Appearance: | dark blue powder / solution |
| Molecular weight: | 601.22 |
| Molecular formula: | C35H45ClN6O |
| Solubility: | soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |