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| Synonyms | MonoclonalCCNE1antibody,Anti-CCNE1antibody,CCNE-1antibody,CCNEantibody,CCNE1,CyclinE1antibody,CCNE1antibody,CCNE-1,CCNE1 |
|---|---|
| Specificity | HumanCCNE1 |
| CrossReactivity | Human.Otherspeciesnotstudied |
| Applications | DotBlot,WB |
| Immunogen | CCNE1antibodywasraisedinmouseusingrecombinantHumanCyclinE1(Ccne1) |
| Host | Mouse |
|---|---|
| Clone | 2121C1a |
| Isotype | IgG1 |
| MethodofPurification | CCNE1antibodywaspurifiedusingproteinGcolumnchromatographyfromculturesupernatantofhybridomaculturedinamediumcontainingbovineIgG-depleted(approximately95%)fetalbovineserum. |
| Form&Buffer | CCNE1antibodyinPBS(3.0mMKCl,1.5mMKH2PO4,140mMNaCl,8.0mMNa2HPO4(pH7.4))containing1%bovineserumalbumin(BSA)and0.05%sodiumazide(NaN3). |
| Concentration | 100ug/ml |
| Storage | Storeat2-8degCforuptooneyear.Werecommendlongtermstorageat-20degC.Avoidrepeatedfreezingandthawing. |
|---|---|
| BiohazardInformation | Thisantibodysolutioncontainssodiumazide(NaN)asapreservative.ThereisapotentialhazardthatNaNreactswithcopperorleadtoproduceanexplosivecompound.Forsafedisposal,thevialhastobewashedthoroughlywithwater.Itisstronglyadv |
| UsageRecommendations | WB:0.2-2ug/ml |
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| BIOLOGicalSignificance | Theproteinencodedbythisgenebelongstothehighlyconservedcyclinfamily,whosemembersarecharacterizedbyadramaticperiodicityinproteinabundancethroughthecellcycle.CyclinsfunctionasregulatorsofCDKkinases.Differentcyclinsexhibitdistinctexpressionanddegradationpatternswhichcontributetothetemporalcoordinationofeachmitoticevent.ThiscyclinformsacomplexwithandfunctionsasaregulatorysubunitofCDK2,whoseactivityisrequiredforcellcycleG1/Stransition.ThisproteinaccumulatesattheG1-SphaseboundaryandisdegradedascellsprogressthroughSphase.Overexpressionofthisgenehasbeenobservedinmanytumors,whichresultsinchromosomeinstABIlity,andthusmaycontributetotumOrigenesis. |
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