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AntibodiesOnline/Platform Reagent/ABIN1889497/100 μg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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AntibodiesOnline/Platform Reagent/ABIN1889497/100 μg

Application
TransfectedCellCulture(TCC)
Options
Bulkdiscount
Supplier
SupplierProductNo.
PurposeForfastandconvenientcharacterizationofinteractionsbetweenGFP-andRFP-taggedproteinsinlivemammaliancellsbyconventionalfluorescencemicroscopy.
BrandF2H®
CharacteristicstransfectionsupplementenablingassemblyofthenuclearPPI-platform
Comment

Intracellularanalysisofprotein-proteininteractions(PPIs)iscrucialforunderstandingofintimaterelationshipsofproteinswithintheirnativecellularenvironment.Besides,cell-basedanalysisisoftenrequiredtosupportbiochemicaldataobtainedwithinvitroPPIassays.
WiththeFluorescentTwo-Hybrid(F2H®)Kit,evaluationofselectedPPIscanberapidlycarriedoutinlivemammaliancells.Interactionsarevisualizedbysimplefluorescencemicroscopyasco-localizationofgreen-andredfluorescentsignalsataPPI-platforminthenucleusofF2H®-Cells,transientlyco-transfectedwithinteractingGFP-andRFP-taggedproteins.

RestrictionsForResearchUseonly
Concentration1mg/mL
Storage4°C/-20°C
StorageCommentStorePlatformReagentat+4°C(shortterm)orin-20°C(longterm).
ExpiryDate6months
SupplierImages
 image for Platform Reagent (ABIN1889497)F2H®principle:-F2Hcells(BHKorigen)carryaGFP-anchoringplatforminthenucleu...
 image for Platform Reagent (ABIN1889497)F2H®result:ExemplaryF2Himagesobtainedbyfluorescencemicroscopy
Left,inte...
 image for Platform Reagent (ABIN1889497)Anoverviewimageofco-transfectedF2H®-Cellsat20Xmagnification.CellswerePFA-f...
 image for Platform Reagent (ABIN1889497)F2H®-Cellswereco-transfectedwithinteractingproteins,fixed,stainedwithanucle...
Productcitedin:Yurlova,Derks,Buchfellner,Hickson,Janssen,Morrison,Stansfield,Brown,Ghadessy,Lane,Rothbauer,Zolghadr,Krausz:"Thefluorescenttwo-hybridassaytoscreenforprotein-proteininteractioninhibitorsinlivecells:targetingtheinteractionofp53withMdm2andMdm4."in:Journalofbiomolecularscreening,Vol.19,Issue4,pp.516-25,2014(PubMed).

Wolf,Mantri,Heim,Müller,Fichter,Mackeen,Schermelleh,Dadie,Leonhardt,Vénien-Bryan,Kessler,Schofield,Böttger:"Thepolyserinedomainofthelysyl-5hydroxylaseJmjd6mediatessubnuclearlocalization."in:TheBiochemicaljournal,Vol.453,Issue3,pp.357-70,2013(PubMed).

Brown,Quah,Jong,Goh,Chiam,Khoo,Choong,Lee,Yurlova,Zolghadr,Joseph,Verma,Lane:"Stapledpeptideswithimprovedpotencyandspecificitythatactivatep53."in:ACSchemicalbiology,Vol.8,Issue3,pp.506-12,2013(PubMed).

Wei,Joseph,Sim,Yurlova,Zolghadr,Lane,Verma,Ghadessy:"InvitroselectionofmutantHDM2resistanttoNutlininhibition."in:PLoSONE,Vol.8,Issue4,pp.e62564,2013(PubMed).

Mortusewicz,Fouquerel,Amé,Leonhardt,Schreiber:"PARGisrecruitedtoDNAdamagesitesthroughpoly(ADP-ribose)-andPCNA-dependentmechanisms."in:Nucleicacidsresearch,Vol.39,Issue12,pp.5045-56,2011(PubMed).


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