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| 细胞形态： | 上皮样 |
| ATCCNumber： | CL-188™ |
| 相关疾病： | 大肠癌 |
| 生长状态： | 贴壁生长 |
| 运输方式： | 冻存运输 |
| 数量： | 大量 |
| 器官来源： | 结肠 |
| 是否是肿瘤细胞： | 1 |
| 物种来源： | 人 |
| 年限： | Dukes'typeB |
| Designations: | LS174T | ||
| Depositors: | NorthwesternUniversity | ||
| BiosafetyLevel: | 1 | ||
| Shipped: | frozen | ||
| Medium&Serum: | SeePropagation | ||
| GrowthProperties: | adherent | ||
| Organism: | Homosapiens | ||
| Morphology: | epithelial | ||
| Source: | Organ:colon TumorStage:DukestypeB Disease:colorectaladenocarcinoma | ||
| CellularProducts: | carcinoembryonicantigen(CEA)(TheproductionofCEAintheATCCseedstockwas1944ngper10(6)cellsin10days.) interleukin10(IL-10)[22511] interleukin6(IL-6) mucin | ||
| Permits/Forms: | InadditiontotheMTAmentionedabove,otherATCCand/orregulatorypermitsmayberequiredforthetransferofthisATCCmaterial.AnyonepurchasingATCCmaterialisultimatelyresponsibleforobtainingthepermits.Pleaseclickhereforinformationregardingthespecificrequirementsforshipmenttoyourlocation. | ||
| Tumorigenic: | Yes | ||
| Oncogene: | myc+;myb+;ras+;fos+;p53+;sis-;abl-;ros-;src- | ||
| AntigenExpression: | serologicallydefinedcoloncancerantigen3;Homosapiens,expressed HLAA2,B13,B50;BloodtypeO | ||
| DNAProfile(STR): | Amelogenin:X CSF1PO:10,13,14 D13S317:10 D16S539:11,13 D5S818:11,15 D7S820:11 THO1:6,7 TPOX:8,9 vWA:15,17 | ||
| CytogeneticAnalysis: | 45,X;oneXchromosomemissing;nootherchromosomalaberrations | ||
| Age: | 58years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | LS174TisavariantofLS180(ATCCCL-187)thathasbeenmaintainedbyusingtrypsininthesubcultureprotocol.Itismoreeasilysubcultivatedthanthatparentlineand,likeLS180,itisreportedtoproducelargeamountsofcarcinoembryonicantigen(CEA). Electronmicroscopicstudiesrevealedabundantmicrovilliandintracytoplasmicmucinvacuoles[PubmedID:1262041]. Theyarenegativeforp53antigenexpression,butpositiveformRNAexpression. LS174Tcellsstainpositivelyforcytokeratins. Thelineispositiveforexpressionofc-myc,N-myc,H-ras,N-ras. Myb,andfosoncogenes. K-rasandsisoncogeneexpressionwerenotdetected. | ||
| Propagation: | ATCCcompletegrowthmedium:ThebasemediumforthiscelllineisATCC-formulatedEaglesMinimumEssentialMedium,CatalogNo.30-2003.Tomakethecompletegrowthmedium,addthefollowingcomponentstothebasemedium:fetalbovineserumtoafinalconcentrationof10%. Atmosphere:air,95%;carbondioxide(CO2),5% Temperature:37.0°C | ||
| Subculturing: | Protocol:
| ||
| Preservation: | Freezemedium:Completegrowthmediumsupplementedwith5%(v/v)DMSO Storagetemperature:liquidnitrogenvaporphase | ||
| RelatedProducts: | Recommendedmedium(withouttheadditionalsupplementsorserumdescribedunderATCCMedium):ATCC30-2003 recommendedserum:ATCC30-2020 derivedfromsameindividual:ATCCCL-187 | ||
| References: | 2148:TomBH,etal.Humancolonicadenocarcinomacells.I.Establishmentanddescriptionofanewline.InVitro12:180-191,1976.PubMed:1262041 3524:TomBH,etal.Processofproducingcarcinoembryonicantigen.USPatent4,228,236datedOct141980 22147:ChenTR,etal.Intercellularkaryotypicsimilarityinnear-diploidcelllinesofhumantumororigins.CancerGenet.Cytogenet.10:351-362,1983.PubMed:6652615 22511:GastlGA,etal.Interleukin-10productionbyhumancarcinomacelllinesanditsrelationshiptointerleukin-6expression.Int.J.Cancer55:96-101,1993.PubMed:8344757 22861:TrainerDL,etal.Biologicalcharacterizationandoncogeneexpressioninhumancolorectalcarcinomacelllines.Int.J.Cancer41:287-296,1988.PubMed:3338874 32265:TsaoH,etal.Novelmutationsinthep16/CDKN2AbindingregionoftheCyclin-dependentKinase-4gene.CancerRes.58:109-113,1998.PubMed:9426066 32377:ShibataD,etal.Genomicinstabilityinrepeatedsequencesisanearlysomaticeventincolorectaltumorigenesisthatpersistsaftertransformation.Nat.Genet.6:273-281,1994.PubMed:8012390 32794:KutcheraW,etal.ProtaglandinHsynthase2isexpressedabnormallyinhumancoloncancer:evidenceforatranscriptionaleffect.Proc.Natl.Acad.Sci.USA93:4816-4820,1996.PubMed:8643486 | ||