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| ProductName | Wilm’sTumorSuppressor1(-KTS) |
| Size | 5,000U |
| Description | WT-1,theproductofWilms’tumorsuppressorgeneWt1,isanuclearproteinwithstructuralmotifscharacteristicoftranscriptionfactors,includingfourC-terminalzincfingers.Whiledifferentpre-mRNAprocessingcouldresultin16isoformsoftheprotein,inclusionorexclusionofexon5andthethreeaminoacids(KTS)betweenzincfingers3and4largelyaffectstheactivityofWT1protein(1-3).Suchacomplexpost-transcriptionalregulation,particularlyinsplicing,mayrepresentamajorregulatorymechanismfortumorigenesisoftheWilms’tumor.WT1(-KTS)appearstohavedifferentbindingaffinitytobothDNAandRNAcomparingtothe+KTSform(4,5). TheN-terminalFlag-taggedWT-1protein(residue1-446,includingexon5butwithoutKTS)wasexpressedinabaculovirussystemandpurifiedbyaffinityandFPLCchromatography. |
| Applications | invitrofunctionstudiesincluding
|
| Source | Baculovirus-insectcell |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofprotein.2-10unitsaresufficientforagelmobilityshiftassayina20µlreaction.50-200unitsarerequiredforareconstitutedtranscriptionassayand100unitsaresufficientforaprotein-proteininteractionassay. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 7490 |
| Accession# | NM_000378 |
| GeneName | Wilmstumor1 |
| GeneSymbol | WT-1 |
| GeneSynonyms | GUD;AWT1;WAGR;WT33;NPHS4;WIT-2;EWS-WT1 |
| MW | 55.7kDa |
| ProteinSequence | 1MQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRG 61ASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLG 121GPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPF 181GPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFP 241NHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQ 301LECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHG 361VFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKP 421YQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFS 481CRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Haber,DA.etal.,(1991)Proc.Natl.Acad.Sci.USA88,9618-9622;(1993)Science262,2057-2059 2.Kreidberg,JA.etal.,(1993)Cell74,679-691 3.Larsson,SH.etal.,(1995)Cell81,391-401 4.Little,M.etal.,(1997)Hum.Mutat.9,209-225 5.Englert,C.etal.,(1995)EMBOJ.14,4662-4675 |