TIB161 HuT 78 人T淋巴细胞白血病细胞

TIB-161HuT78人T淋巴细胞白血病细胞
ATCC®Number: TIB-161™       
Designations: HuT78 
Depositors:  AFGazdar 
BiosafetyLevel:1 
Shipped: frozen 
Medium&Serum: SeePropagation 
GrowthProperties:suspension
Organism:Homosapiens(human) 
Morphology:lymphoblast

 
Source:Disease:SezarySyndrome
CellType:cutaneousTlymphocyte;
CellularProducts:interleukin2[1140]
tumornecrosisfactoralpha(TNFalpha)[23420] 
Permits/Forms:InadditiontotheMTAmentionedabove,otherATCCand/orregulatorypermitsmayberequiredforthetransferofthisATCCmaterial.AnyonepurchasingATCCmaterialisultimatelyresponsibleforobtainingthepermits.Pleaseclickhereforinformationregardingthespecificrequirementsforshipmenttoyourlocation. 
 
Applications:transfectionhost(NucleofectiontechnologyfromLonza)
Receptors:interleukin2(IL-2),expressed[1140]
Tumorigenic:Yes 
AntigenExpression:CD4;Homosapiens[22610]
DNAProfile(STR):Amelogenin:X,Y
CSF1PO:11,12
D13S317:8,12
D16S539:11,12
D5S818:11,12
D7S820:8,11
THO1:8,9
TPOX:8,9
vWA:14,15
Age: 53yearsadult 
Gender: male 
Ethnicity: Caucasian 
Comments:H9(ATCCHTB-176)isaclonalderivativeofHuT78[Pubmed:2567177].
Propagation: ATCCcompletegrowthmedium:ThebasemediumforthiscelllineisATCC-formulatedIscovesModifiedDulbeccosMedium,CatalogNo.30-2005.Tomakethecompletegrowthmedium,addthefollowingcomponentstothebasemedium:fetalbovineserumtoafinalconcentrationof20%.
Atmosphere:air,95%;carbondioxide(CO2),5%
Temperature:37.0°C
Subculturing: Protocol:Culturescanbemaintainedbyadditionoffreshmediumorreplacementofmedium.Alternatively,culturescanbeestablishedbycentrifugationwithsubsequentresuspensioninfreshmediumat2X10exp5viablecells/ml.Maintainculturesatcellconcentrationsbetween5X10exp4and8X10exp5viablecells/ml.maintaincelldensityatlessthan1X10exp6cells/ml.
MediumRenewal:Twotothreetimesweekly
Preservation: Freezemedium:Completegrowthmedium95%;DMSO,5%
Storagetemperature:liquidnitrogenvaporphase
DoublingTime: about65hours 
RelatedProducts:Recommendedmedium(withouttheadditionalsupplementsorserumdescribedunderATCCMedium):ATCC30-2005
recommendedserum:ATCC30-2020
derivative:ATCCHTB-176
References:1140:GootenbergJE,etal.HumancutaneousTcelllymphomaandleukemiacelllinesproduceandrespondtoTcellgrowthfactor.J.Exp.Med.154:1403-1418,1981.PubMed:6975346
22484:MannDL,etal.OriginoftheHIV-susceptiblehumanCD4+celllineH9.AIDSRes.Hum.Retroviruses5:253-255,1989.PubMed:2567177
22610:GazdarAF,etal.MitogenrequirementsfortheinvitropropagationofcutaneousT-celllymphomas.Blood55:409-417,1980.PubMed:6244013
23228:ChenTR.KaryotypicderivationofH9celllineexpressinghumanimmunodeficiencyvirussusceptibility.J.Natl.CancerInst.84:1922-1926,1992.PubMed:1460674
23420:OConnellMA,etal.CellularproliferationandactivationofNFkappaBareinducedbyautocrineproductionoftumornecrosisfactoralphainthehumanTlymphomalineHuT78.J.Biol.Chem.270:7399-7404,1995.PubMed:7706285
32283:HuSX,etal.Developmentofanadenovirusvectorwithtetracycline-regulatablehumantumornecrosisfactoralphageneexpression.CancerRes.57:3339-3343,1997.PubMed:9269991
32396:KolanusW,etal.alphaLbeta2integrin/LFA-1bindingtoICAM-1inducedbycytohesin-1acytoplasmicregulatorymolecule.Cell86:233-242,1996.PubMed:8706128
32796:BloomTJ,BeavoJA.Identificationandtissue-specificexpressionofPDE7phosphodiesterasesplicevariants.Proc.Natl.Acad.Sci.USA93:14188-14192,1996.PubMed:8943082