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| ProductName | MDM2(Mousedoubleminute2)HumanOncoprotein |
| Size | 10,000U |
| Description | OriginallydiscoveredasoneofthreegenesamplifiedondoubleminutechromosomesinatumorigenicderivativeofNIH3T3cells(1),MDM2waslatershowntopossessoncogenicpotentialwhenoverexpressed(2,3).High-levelexpressionofMDM2hasalsobeenshowntoconfertumorigenicpotentialuponnontransformedrodentfibroblastsinathymicnudemice(2,3).MDM2canimmortalizeratembryofibroblastsandcancooperatewithactivatedRAStotransformthesecells(3).ElevatedlevelsofMDM2proteinhavebeenfoundinavarietyofhumantumors,mostnotablyinsofttissuesarcomaswhereupto30%ofprimarytumorscontainmultiplecopiesoftheMDM2gene(4).OnemechanismbywhichMDM2overexpressionpromotestumordevelopmentisthroughitsabilitytobindtothep53tumorsuppressor,therebyblockingthetransactivation(5-7),cellcyclearrest(8),andapoptoticfunctionsofp53(9).MDM2caninhibitp53activityinanumberofwaysincludingpreventingp53fromrecruitingTAFs,promotingnuclearexport,inhibitingp53acetylation,andperhapsmostimportantlybyvirtueofitsfunctionasanE3ubiquitinligasewithspecificityfor,amongothers,p53(reviewedin10).Inadditiontoregulatingp53levelsbytargetingp53forproteasomaldegradationMDM2alsotransfersubiquitintoitself,MDMX,theß2adrenergicreceptor,glucocorticoidreceptor,TIP60,andPCAF(10). |
| Applications | invitrotranscriptionassays,DNA-proteinandprotein-proteininteractionassays |
| Source | E.coli |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 4193 |
| Accession# | NM_002392 |
| GeneName | MDM2oncogene,E3ubiquitinproteinligase |
| GeneSymbol | MDM2 |
| GeneSynonyms | HDMX;hdm2;ACTFS |
| MW | 57.8kDa |
| ProteinSequence | 1mvrsrqmcntnmsvptdgavttsqipaseqetlvrpkplllkllksvgaqkdtytmkevl 61fylgqyimtkrlydekqqhivycsndllgdlfgvpsfsvkehrkiytmiyrnlvvvnqqe 121ssdsgtsvsenrchleggsdqkdlvqelqeekpssshlvsrpstssrrraiseteensde 181lsgerqrkrhksdsislsfdeslalcvireiccerssssestgtpsnpdldagvsehsgd 241wldqdsvsdqfsvefevesldsedyslseegqelsdeddevyqvtvyqagesdtdsfeed 301peisladywkctscnemnpplpshcnrcwalrenwlpedkgkdkgeisekaklenstqae 361egfdvpdckktivndsrescveenddkitqasqsqesedysqpstsssiiyssqedvkef 421ereetqdkeesvesslplnaiepcvicqgrpkngcivhgktghlmacftcakklkkrnkp 481cpvcrqpiqmivltyfp |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Cahilly-Snyder,L.,etal.,(1987)Somat.CellMol.Genet.13:235-244. 2.Fakharzadeh,S.etal.,(1991)EMBOJ.10:1565-1569 3.Finlay,C.A.,(1993)Mol.Cell.Biol.13:301-306 4.Leach,F.S.,etal.,(1993)CancerRes.53:2231-2234. 5.Momand,J.,etal.,(1992)Cell69:1237-1245. 6.Oliner,J.D.,etal.,(1992)Nature358:80-83 7.Oliner,J.D.,etal.,(1993)Nature362:857-860 8.Chen,J.,etal.,(1996)Mol.Cell.Biol.16:2445-2452. 9.Dornan,D.,etal.,(2004)Nature429:86-92 10.BondGL,etal.,(2005)CurrentCancerDrugTargets.5(1):3-8. |