SiHa细胞来源

产品简介

SiHa细胞应如何避免细胞污染，细胞污染的种类可分成细菌、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。SiHa细胞何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上之更换时间，按时更换培养基即可。

产品详细信息 SiHa细胞 细胞形态： 上皮样 年限：gradeII是否是肿瘤细胞：1物种来源：人器官来源：宫颈ATCCNumber：HTB-35™数量：大量相关疾病：鳞状细胞癌运输方式：冻存运输生长状态：贴壁生长SiHa细胞Designations:SiHaDepositors:YItoBiosafetyLevel:2[Cellscontainhumanpapillomavirus]Shipped:frozenMediumSerum:SeePropagationGrowthProperties:adherentOrganism:HomosapiensMorphology:epithelialSource:Organ:cervixTumorStage:gradeIIDisease:squamouscellcarcinomaPermits/Forms:InadditiontotheMTAmentionedabove,otherATCCand/orregulatorypermitsmayberequiredforthetransferofthisATCCmaterial.SiHa细胞AnyonepurchasingATCCmaterialisultimatelyresponsibleforobtainingthepermits.Pleaseclickhereforinformationregardingthespecificrequirementsforshipmenttoyourlocation.Applications:transfectionhost(RocheTransfectionReagents)Tumorigenic:YesOncogene:p53+;pRB+DNAProfile(STR):Amelogenin:XCSF1PO:12D13S317:11D16S539:12D5S818:9D7S820:10THO1:6,9TPOX:8vWA:14,17CytogeneticAnalysis:SiHa细胞modalnumber=69;range=51to72.Thisisahypertriploidhumancelllinewiththemodalchromosomenumberof71,occurringin24%ofcells.Mostcellshadthechromosomenumbersdistributedbetween69and72.Polyploidcellsoccurredat7.6%.FifteenormoreMarkerchromosomeswerecommontomostcells.Amongthemaredup(2)(q22q31)anddel(2)(q31)whichprobablyresultedfromthebalancedtranslocationbetweentwoN2s.Mostcellshadtwocopiesofdel(2).M2isanA3-sizedacrocentric.M13isaminutesubmetacentricwith1-3copiespercell.OriginsofbothM2andM13arenotidentified.ThereweretwocopiesofnormalXchromosomes.N2wasabsentandprobablywasreplacedbydup(2)anddel(2).Isoenzymes:AK-1,1ES-D,2G6PD,BGLO-I,2Me-2,1PGM1,1PGM3,1Age:55years*****Gender:femaleEthnicity:AsianComments:SiHa细胞ThislinewasestablishedfromfragmentsofaprimarytissuesampleobtainedaftersurgeryfromaJapanesepatient.Electronmicroscopicobservationsrevealedpresenceoftypicaldesmosomesatthecelljunctionsandanabundanceoftonofilamentsinthecytoplasm.Mycoplasmacontaminationwasdetectedandeliminatedin1975.Thelineisreportedtocontainanintegratedhumanpapillomavirustype16genome(HPV-16,1to2copiespercell).Propagation:ATCCcompletegrowthmedium:ThebasemediumforthiscelllineisATCC-formulatedEagle"sMinimumEssentialMedium,CatalogNo.30-2003.Tomakethecompletegrowthmedium,addthefollowingcomponentstothebasemedium:fetalbovineserumtoafinalconcentrationof10%.Atmosphere:air,95%;carbondioxide(CO2),5%Temperature:37.0°CSubculturing:Protocol:Removeanddiscardculturemedium.Brieflyrinsethecelllayerwith0.25%(w/v)Trypsin-0.53mMEDTAsolutiontoremovealltracesofserumthatcontainstrypsininhibitor.Add2.0to3.0mlofTrypsin-EDTAsolutiontoflaskandobservecellsunderaninvertedmicroscopeuntilcelllayerisdispersed(usuallywithin5to15minutes).Note:Toavoidclumpingdonotagitatethecellsbyhittingorshakingtheflaskwhilewaitingforthecellstodetach.Cellsthataredifficulttodetachmaybeplacedat37�Ctofacilitatedispersal.Add6.0to8.0mlofcompletegrowthmediumandaspiratecellsbygentlypipetting.Addappropriatealiquotsofthecellsuspensiontonewculturevessels.Incubateculturesat37�C.SubcultivationRatio:Asubcultivationratioof1:3to1:8isrecommendedMediumRenewal:2to3timesperweekPreservation:Freezemedium:Completegrowthmediumsupplementedwith5%(v/v)DMSOStoragetemperature:liquidnitrogenvaporphaseRelatedProducts:Recommendedmedium(withouttheadditionalsupplementsorserumdescribedunderATCCMedium):ATCC30-2003recommendedserum:ATCC30-2020References:22565:BakerCC,etal.Structuralandtranscriptionalanalysisofhumanpapillomavirustype16sequencesincervicalcarcinomacelllines.J.Virol.61:962-971,1987.PubMed:302943022995:PaterMM,PaterA.Humanpapillomavirustypes16and18sequencesincarcinomacelllinesofthecervix.Virology145:313-318,1985.PubMed:299215323180:YeeC,etal.Presenceandexpressionofhumanpapillomavirussequencesinhumancervicalcarcinomacelllines.Am.J.Pathol.119:361-366,1985.PubMed:299021723192:FriedlF,etal.Studiesonanewhumancellline(SiHa)derivedfromcarcinomaofuterus.I.Itsestablishmentandmorphology.Proc.Soc.Exp.Biol.Med.135:543-545,1970.PubMed:552959823324:ScheffnerM,etal.Thestateofthep53andretinoblastomagenesinhumancervicalcarcinomacelllines.Proc.Natl.Acad.Sci.USA88:5523-5527,1991.PubMed:164821829988:HendricksDT,etal.FHITgeneexpressioninhumanovarian,endometrial,andcervicalcancercelllines.CancerRes.57:2112-2115,1997.PubMed:918710532270:OlivePL,BanathJP.Multicellspheroidresponsetodrugspredictedwiththecometassay.CancerRes.57:5528-5533,1997.PubMed:9407963