AntibodiesOnline/GID Complex Subunit 8 Homolog (S. Cerevisiae) (GID8) (AA 1228) protein (His tag)/

ProteinName

ProteinCharacteristics

AA1-228

Origin

Mouse(Murine)

Source

Escherichiacoli(E.coli)

ProteinType

Recombinant

Purificationtag/Conjugate

ThisGID8proteinislabelledwithHistag.

Application

Crystallization(Crys),ELISA,SDS-PAGE(SDS),WesternBlotting(WB)

Options

Supplier

SupplierProductNo.

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Sequence	MSYAEKPDEITKDEWMEKLNNLHVQRADMNRLIMNYLVTEGFKEAAEKFRMESGIEPSVDLETLDERIKIREMILKGQIQEAIALINSLHPELLDTNRYLYFHLQQQHLIELIRQRETEAALEFAQTQLAEQGEESRECLTEMERTLALLAFDSPEESPFGDLLHMMQRQKVWSEVNQAVLDYENRESTPKLAKLLKLLLWAQNELDQKKVKYPKMTDLSKGVIEEPK Sequencewithouttag.Taglocationisatthediscretionofthemanufacturer.Ifyouhaveaspecialrequest,pleasecontactus.
Characteristics	MadeinGermany-fromdesigntoproduction-byhighlyexperiencedproteinexperts. MouseGid8Protein(raisedinE.Coli)purifiedbymulti-step,protein-specificprocesstoensurecrystallizationgrade. State-of-the-artalgorithmusedforplasmiddesign(Genesynthesis).
Purification	Twosteppurificationofproteinsexpressedinbacterialculture: Inafirstpurificationstep,theproteinispurifiedfromtheclearedcelllysateusingthreedifferentHis-tagcapturematerials:highyield,EDTAresistant,orDTTresistant.EluatefractionsareanalyzedbySDS-PAGE. Proteincontainingfractionsofthebestpurificationaresubjectedtosecondpurificationstepthroughsizeexclusionchromatography.EluatefractionsareanalyzedbySDS-PAGEandWesternblot.
Purity	>95 %asdeterminedbySDSPAGE,SizeExclusionChromatographyandWesternBlot.
Sterility	0.22μmfiltered
EndotoxinLevel	Endotoxinhasnotbeenremoved.Pleasecontactusifyourequireendotoxinremoval.
Grade	Crystallographygrade

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MolecularWeight	27.7kDaIncludingtag.
UniProt	Q9D7M1

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ApplicationNotes	Inadditiontotheapplicationslistedaboveweexpecttheproteintoworkforfunctionalstudiesaswell.Astheproteinhasnotbeentestedforfunctionalstudiesyetwecannotofferaguranteethough.
Comment	Proteinhasnotbeentestedforactivityyet.Incasesinwhichitishighlylikelythattherecombinantproteinwiththedefaulttagwillbeinsolubleourproteinlabmaysuggestahighermolecularweighttag(e.g.GST-tag)insteadtoincreasesolubility.Wewilldiscussallpossibleoptionswithyouindetailtoassurethatyoureceiveyourproteinofinterest.
Restrictions	ForResearchUseonly

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Format	Liquid
Buffer	100mMNaCL,20mMHepes,10%glycerol.pHvalueisatthediscretionofthemanufacturer.
HandlingAdvice	Avoidrepeatedfreeze-thawcycles.
Storage	-80°C
StorageComment	Storeat-80°C.
ExpiryDate	Unlimited(ifstoredproperly)

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SupplierImages	„CrystallographyGrade“proteinduetomulti-step,protein-specificpurificationprocess

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MolecularWeight	27.7kDaIncludingtag.
UniProt	Q9D7M1