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AntibodiesOnline/DMPD Antioxidant Capacity Assay Kit/ABIN3172701/200 tests188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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AntibodiesOnline/DMPD Antioxidant Capacity Assay Kit/ABIN3172701/200 tests
DetectionRange
10-150μg/mL
MinimumDetectionLimit
10μg/mL
Application
BiochemicalAssay(BCA)
Options
Bulkdiscount
Supplier
SupplierProductNo.
PurposeThekitisdesignedfortheantioxidantcapacitymeasurementinfoods,beveragesandbiologicalsamples.
SampleTypeBeverages,Food,BiologicalSamples
DetectionMethodColorimetric
SpecificitySpecificforantioxidantactivitydetermination.
CharacteristicsDMPDassaykitisrecommendedforantioxidantmeasurementsofbeverages.Thiskitmeasurestheantioxidantactivityofcompoundsthatareabletotransferhydrogenatoms.WhenthecompoundN,N-dimethyl-?-phenylenediamine(DMPD)isinthepresen
ComponentsPlateandthereagentsneccesarytoperformtheassay.
MaterialnotincludedPipettes,reactiontubes,platereader.
SampleVolume20μL
AssayTime15min
PlateUncoated
ReagentPreparation

Reagentspreparation:1.Forstandardsolutionaddexactly1 mLofdeionizedwaterinstandardvial.Keepitat-20 °C.Then,diluteit100timestoperformthecalibrationcurve.2.Dissolve10 μLofstandardsolutionin1000 μLofdeionizedwater.3.Maintaintherestofthestandardsolutionat-20 °C.4.ForDMPD•+solutionformation,mix25 mLofDPMDsolutionina25 mLtube(notprovided)withonevialofReagentA(thismixtureisstablefor12hatroomtemperature).

SamplePreparation

Samplepreparation:Ifyoursampleiscoloured,diluteittoanabsorbancevaluelowerthanA0.

AssayProcedure

StandardPreparationAntioxidantactivityisexpressedasCEAC(VitaminCequivalentsantioxidantcapacity).Forthispurpose,usethe1:100dilutedstandardpreviouslyprepared(seeReagentspreparation).Preparecalibrationcurvesin1 mLtubesasshowninTable1(seekitbooklet).Assay:1.Add20 μLofthesampleorstandardineachwell.2.Add280 μLofDMPD•+solution(seeReagentspreparation)ineachwell.3.Mixthemixtureat25 °Cfor10 minutesundercontinuousstirring.4.Readtheabsorbanceat553nm.

CalculationofResults

1.Calculatetheabsorbanceat553nmaspercentageoftheabsorbanceoftheuninhibitedradicalcationsolution(Blank)accordingtotheequation:InhibitionofA553(%)=(1-Af/A0)x100WhereA0istheabsorbanceofuninhibitedradicalcationandAfistheabsorbancemeasured10 minaftertheadditionofantioxidantsamples.2.Plottheinhibitionofstandardsasafunctionoftheirfinalconcentrations(Table1inbooklet).SeeFigure2foratypicalstandardcurve(seeimages).3.CalculatetheCEACvalueofthesamplesusingtheequationobtainedfromthelinearregressionofthestandardcurvesubstitutedinhibitionpercentagevaluesforeachsample.CEAC(μg/ml)=(sampleinhibitionA553-intercept)/slope

RestrictionsForResearchUseonly
PreservativeSodiumazide
PrecautionofUseThisproductcontainsSodiumazide:aPOISONOUSANDHAZARDOUSSUBSTANCEwhichshouldbehandledbytrainedstaffonly.
Storage4°C/-20°C
StorageCommentDMPDSolution:4°C,DMPDStandard:4°C,DMPDReagentA:-20°C
SupplierImages
Biochemical Assay (BCA) image for DMPD Antioxidant Capacity Assay Kit (ABIN3172701)Typicalstandardcurve

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