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AntibodiesOnline/Mouse antiHuman IgE (Fc Region) Antibody/ABIN363696/1 mg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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AntibodiesOnline/Mouse antiHuman IgE (Fc Region) Antibody/ABIN363696/1 mg
Antigen
IgE
Epitope
FcRegion
135FcRegion
60Chainepsilon
53HeavyChain
7AA245-275
1AA246-275
1Ce2Domain
1Chainepsilon2
1Chainepsilon3
1LightChain
Reactivity
Human
483Human
141Mouse(Murine)
46Dog(Canine)
44Rat(Rattus)
6Monkey
6RhesusMonkey
3Horse(Equine)
2GuineaPig
2Hamster
2Rabbit
1Cow(Bovine)
1Goat
1Sheep(Ovine)
Host
Mouse
290Mouse
286Goat
67Rat
52Rabbit
10Pig
6Sheep
3Human
1Chicken
1Synthetic
Clonality(Clone)
Monoclonal(Le27-NBS01)
Application
FunctionalStudies(Func),FlowCytometry(FACS),ELISA,WesternBlotting(WB)
Options
Bulkdiscount
Supplier
SupplierProductNo.
ImmunogenpurifiedhumanmyelomaIgE
CloneLe27-NBS01
IsotypeIgG1kappa
SpecificityHumanIgE,Fce4,nocross-ractivitywithotherhumanormurineAbisotypes
CharacteristicsIGHE,chromosome14q32.33
PurificationAffinity-purifiedfromcellculturesupernatantbyImmunoaffinityChromatographyonCH-Sepharose4B
Purity>95%,testedbyanalyticalGelPermeationChromatography
SterilitySterilefiltered
BackgroundTheantibodyrecognizesanepitopewithintheCe4domainofhumanIgE.ItdoesnotblockthebindingofIgEtoitshighaffinityreceptoronmastcellsandbasophilsandisthereforesuitabletodetectreceptorboundIgE.Itcanalsobeusedascross-linkingagentforallergen-independenttriggeringofmastcellsandbasophils.
ResearchAreaImmunology,SecondaryAntibodies
ApplicationNotesForfunctionalcellularassays(mastcell/basophilactivation),inparticularwhenusedatdilutions<1:100Qualityassurance:Reactivity>99%,comparedtocalibrator(InternalStandardmAbLe27),testedbyELISAbindingtoimmobilizedhumanStandardIgE(SUS-11)
RestrictionsForResearchUseonly
Concentration1mg/mL
BufferPBS,pH=7.2,sterilefiltered,containsnoNaN3
HandlingAdviceMonoclonalantibodiesshouldnotbestoredatatemperaturebelow-25°Cduetotheaggregationeffectoftheprotein.
Storage4°C
SupplierImages
image for Mouse anti-Human IgE (Fc Region) Antibody (ABIN363696)productpurity:gelpermeationchromatography(Superose12/HR)
image for Mouse anti-Human IgE (Fc Region) Antibody (ABIN363696)Targetbindingkinetics(Biacore
Productcitedin:Rudolf,Zuercher,Nechansky,Ruf,Vogel,Miescher,Stadler,Kricek:"Molecularbasisfornonanaphylactogenicityofamonoclonalanti-IgEantibody."in:Journalofimmunology(Baltimore,Md.:1950),Vol.165,Issue2,pp.813-9,2000(PubMed).

BackgroundpublicationsKnutti-Mueller,Stadler,Magnusson,deWeck:"HumanIgEsynthesisinvitro.Detectionwithmonoclonalantibodies."in:Allergy,Vol.41,Issue6,pp.457-67,1987(PubMed).


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