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AntibodiesOnline/HEK293 Cells IgG isotype control (AVI tag,Biotin,His tag)/ABIN2870555/25 μg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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AntibodiesOnline/HEK293 Cells IgG isotype control (AVI tag,Biotin,His tag)/ABIN2870555/25 μg
Isotype
IgG
Reactivity
Human
Host
HEK-293Cells
217Rabbit
207Mouse
204Goat
169Human
160Rat
127Hamster
112Sheep
93Horse
88Dog
88GuineaPig
88Pig
73Chicken
56Cat(Feline)
50Cow
43Donkey
42Bovine
27SyrianHamster
25ArmenianHamster
24Cat
22Monkey
21Turkey
11GoldenSyrianHamster
8RhesusMonkey
3CynomolgusMonkey(Macacafascicularis)
2HEK-293Cells
2Llama
1CynomolgusMonkey
BiologicalActivity
Active
Conjugate
AVItag,Biotin,Histag
206Biotin
169TexasRed(TR)
167HRP
166Rhodamine
154FITC
108Fluorescein
58AlkalinePhosphatase(AP)
58Peroxidase(POD)
48AgaroseBeads
44PE
19APC
13Cy5
12AlexaFluor488
12AlexaFluor647
11SPRD
9PE-Cy5
9PE-Cy7
6PE-Cy5.5
6PerCP
5AlexaFluor350
5AlexaFluor555
5AlexaFluor594
5AlexaFluor680
5AlexaFluor750
5Allophycocyanin
5Cy3
5Cy5.5
5Cy5.5,PE
5Cy7
5Cy7,PE
5DyLight488
4DyLight350
4DyLight405
4DyLight405LS
4DyLight680
4DyLight755
3AlexaFluor405
3AlexaFluor700
3Cy5.5,APC
3PE-TexasRed
2APC-Cy5.5
2DyLight550
2DyLight650
2Histag
2PE,TexasRed(TR)
1APC,Cy5.5
1APC-Cy7
1AVItag,Biotin
1Cy7,APC
1DyLight594
1PE,Cy5.5
1PE,Cy7
1PerCP-Cy5.5
1SureLight®R-PE
1TRITC
Application
IsotypeControl(IsoC)
Options
Bulkdiscount
Supplier
SupplierProductNo.
BrandMABSol®,PrecisionAvi
IsotypeIgG
FragmentFcfragment
SpecificityBiotinylationofthisproductisperformedusingAvitag™technology.Briefly,thesinglelysineresidueintheAvitagisenzymaticallylabeledwithbiotin.
CharacteristicsThisproteincarriesanAvitag(Avitag™)attheC-terminus,followedbyapolyhistidinetag.TheproteinhasacalculatedMWof28.8kDa.Theproteinmigratesas36kDaonaSDS-PAGEgelunderreducing(R)conditionduetoglycosylationand60-66kDaundernon-reducing(NR)condition.
Purity>95 %asdeterminedbySDS-PAGE.
EndotoxinLevelLessthan1.0EUperμgbytheLALmethod.
BackgroundCrystallizablefragmentscomposedofthecarboxy-terminalhalvesofbothIMMUNOGLOBULINHEAVYCHAINSlinkedtoeachotherbydisulfidebonds.Fcfragmentscontainthecarboxy-terminalpartsoftheheavychainconstantregionsthatareresponsiblefortheeffectorfunctionsofanimmunoglobulin(COMPLEMENTfixation,bindingtothecellmembraneviaFCRECEPTORS,andplacentaltransport).IgG1Fcwasreportedhasanovelroleasapotentialanti-inflammatorydrugfortreatmentofhumanautoimmunediseases.
MolecularWeight28.8kDa
GeneID18,17
UniProtP01857
ResearchAreaSecondaryAntibodies
ApplicationNotesOptimalworkingdilutionshouldbedeterminedbytheinvestigator.
Comment

Ready-to-useAvitagTMbiotinylatedprotein:
TheproductisexclusivelyproducedusingtheAvitagTMtechnology.Briefly,aunique15aminoacidpeptide,theAvitag,isintroducedintotherecombinantproteinduringexpressionvectorconstruction.ThesinglelysineresidueintheAvitagisenzymaticallybiotinylatedbytheE.ColibiotinligaseBirA.

Thissingle-pointenzymaticlabelingtechniquebringsmanyadvantagesforcommonlyusedbindingassays.ThebiotinylationhappensonthelysineresidueofAvitag,andthereforedoesNOTinterferewiththetargetproteinsnaturalbindingactivities.Inaddition,whenimmobilizedonanavidin-coatedsurface,theproteinorientationisuniformbecausethepositionoftheAvitagintheproteinispreciselycontrolled.

RestrictionsForResearchUseonly
FormatLyophilized
ReconstitutionPleaseseeCertificateofAnalysisforspecificinstructions.Forbestperformance,westronglyrecommendyoutofollowthereconstitutionprotocolprovidedintheCoA.
Buffer50mMTris,100mMGlycine, pH7.5
Storage-20°C

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