AntiCorticotropin Releasing Factor抗体(ab8901)

• ProductnameAnti-CorticotropinReleasingFactor抗体
  参阅全部CorticotropinReleasingFactor一抗
• Description
  兔多克隆抗体toCorticotropinReleasingFactor
• TestedapplicationsICC, RIA, WB, NucleotideArray, IHC-Pmoredetails
• Speciesreactivity
  Reactswith: Mouse,Rat,Human
• Immunogen

  Syntheticpeptide,correspondingtoaminoacids1-41ofRatCorticotropinReleasingFactor.

Properties

• FormLiquid
• StorageinstructionsShippedat4°C.Storeat+4°Cshortterm(1-2weeks).Upondeliveryaliquot.Storeat-20°Cor-80°C.Avoidfreeze/thawcycle.
• StoragebufferLiquidantiserum
• PurityWholeantiserum
• ClonalityPolyclonal
• IsotypeIgG
• ResearchAreas
  • Neuroscience
  •  
  • Neurotransmitter
  •  
  • Neuropeptides
  •  
  • Hormones

  • SignalTransduction
  •  
  • GrowthFactors/Hormones
  •  
  • Hormones

  • Neuroscience
  •  
  • Endocrinesystem
  •  
  • Hypothalamicpituitaryadrenalaxis

Applications

Target

• RelevanceCRFprecursorisa41-aminoacidpeptidederivedfroma196-aminoacidpreprohormone.CRFisaneuroendocrinehormonesecretedbytheparaventricularnucleus(PVN)ofthehypothalamusandisakeyplayerinthehypothalamicpituitaryadrenalaxis.Itisalsosynthesizedinperipheraltissues,suchasTlymphocytesandishighlyexpressedintheplacenta.NeuronalCRFisimportantinavarietyofdiverserolesincluding,memory,depression,stress,kidneyfunction,parturition,metabolismandfeeding(tomentionafew).
• CellularlocalizationCytoplasmicandSecreted
• Databaselinks
  • EntrezGene:1392 Human
  • EntrezGene:12918 Mouse
  • EntrezGene:81648 Rat
  • Omim:122560 Human
  • SwissProt:P06850 Human
  • SwissProt:Q8CIT0 Mouse
  • SwissProt:P01143 Rat
  • Unigene:75294 Human

  seeall
• Alternativenames
  • Corticoliberinantibody
  • Corticoliberinprecursorantibody
  • Corticotropinreleasingfactorantibody

  seeall

Anti-CorticotropinReleasingFactorantibodyimages

• 

  Westernblot-Anti-CorticotropinReleasingFactorantibody(ab8901)ThisimageiscourtesyofanAbreviewsubmittedbyJean-ChristopheHelbling
  Anti-CorticotropinReleasingFactorantibody(ab8901)at1/2500dilution+Mousehypothalamuswholetissuelysateat50µg
  
  Secondary
  HRP-conjugatedDonkeyanti-rabbitIgGpolyclonal-undiluted
  developedusingtheECLtechnique
  
  Performedundernon-reducingconditions.
  
  Observedbandsize: 25kDa(whyistheactualbandsizedifferentfromthepredicted?)
  Additionalbandsat: 17kDa(possiblenon-specificbinding),23kDa(possiblenon-specificbinding),35kDa(possiblenon-specificbinding).
  
  Exposuretime: 1minute

  ThisimageiscourtesyofanAbreviewsubmittedbyJean-ChristopheHelbling

  SeeAbreview

• 

  Radioimmunoassay-Anti-CorticotropinReleasingFactorantibody(ab8901)ThisimageisacourtesyofanAnonymousAbreview
  Radioimmunoassayusingab8901 
  -Day1:AddRecombinantProteinA/G(10ug/ml)toa96wellsplate;Parafilmwrap;incubateovernightat4°C. 
  -Day2:AspirateandwashtheplatetoremovelooselyboundProteinA/G;addantibodysolution(dilution1:50000);parafilmwrap;incubate24hat4°C. 
  -Day3:Aspirateandwashtheplatetoremovelooselyboundantibody;addCRFstandard(0,0.03,0.1,0.3,1,3,10,30ng/ml);parafilmwrap;incubate24hat4°C. 
  -Day4:DONOTASPIRATE;add ¹²⁵I-CRF;incubate24hat4°C. 
  -Day5:Aspirateandwashtheplate;breaktheplateandcollectthewellsinscintillationtubes;add2mlofscintillationliquid;countinbetacounter(openthe3Hwindowtoatleast30-40keV),atleast2min/tube.
• 

  Immunohistochemistry(Formalin/PFA-fixedparaffin-embeddedsections)-Anti-CorticotropinReleasingFactorantibody(ab8901)ThisimageiscourtesyofananonymousAbreview
  Immunohistochemicalanalysisofratbraintissue,stainingCorticotropinReleasingFactorwithab8901. 
  
  Tissuewasfixedwithparaformaldehyde,permeabilizedwith0.01%TritonX-100andblockedwith10%serumfor1hourat25°C;antigenretrievalwasbyheatmediationinacitratebuffer(pH6).Sampleswereincubatedwithprimaryantibody(1/100indiluent)for24hoursat4°C.AnAlexaFluor®594-conjugatedgoatanti-rabbitpolyclonalIgG(1/500)wasusedasthesecondaryantibody.Thefigureontheleftisasecondaryonlycontrol.

  SeeAbreview