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AntibodiesOnline/p53 ELISA Kit (Tumor Protein P53)/ABIN2748488/96 tests188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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AntibodiesOnline/p53 ELISA Kit (Tumor Protein P53)/ABIN2748488/96 tests
Antigen
TumorProteinP53(TP53)
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellulartumorantigenp53
  • tumorproteinp53
  • tumorsupressorp53
  • p53tumorsuppressor
  • transformationrelatedprotein53
  • transformationrelatedprotein53,pseudogene
  • trichoplein,keratinfilamentbinding
  • WistarclonepR53P1p53pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Alternatives
Humanp53ELISAKit
Kitswithalternativereactivityto:
85Human
34Mouse(Murine)
32Rat(Rattus)
4Chicken
4Monkey
4Pig(Porcine)
4Rabbit
3Cow(Bovine)
3Dog(Canine)
3GuineaPig
3Sheep(Ovine)
MethodType
CellELISA
Application
ELISA
Options
Bulkdiscount
Supplier
SupplierProductNo.
PurposeCell-BasedHuman/Rat/Mousep53(S15)PhosphorylationELISAKit.Suitableforadherentwholecelllines.
SampleTypeCellCultureCells
AnalyticalMethodSemi-Quantitative
DetectionMethodColorimetric
SpecificityTheantibodiesprovidedinthiskitrecognizesHuman/Rat/Mousep53phosphorylatedatSerine-15andtotalp53forcomparison.
Characteristics
  • Siteandsignalpathway-specific
  • Invitrodetectionofadherentcellculture
  • Nosamplelysisneeded
  • CompatiblewithastandardELISAplatereader
  • FasterresultsthanwithELISA
  • Adaptableforhigh-throughputscreeninganddrugdiscovery
Components
  • uncoated96-wellMicroplate
  • WashBufferA
  • WashBufferB
  • FixingSolution
  • QuenchingBuffer
    • BlockingBuffer
  • Anti-phosphoantibody
  • Anti-panantibody
  • HRP-ConjugatedSecondaryAntibody
  • TMBOne-StepSubstrate
  • StopSolution
    • Materialnotincluded
    • Distilledordeionizedwater
    • 100 mLand1litergraduatedcylinders
    • Tubestopreparesampledilutions
    • ProteaseandPhosphataseinhibitors
    • Precisionpipettestodeliver2 μLto1 mLvolumes
    • Adjustable1-25 mLpipettesforreagentpreparation
    • Benchtoprockerorshaker
    • Microplatereadercapableofmeasuringabsorbanceat450nm
    AlternativeNameP53(TP53ELISAKitAbstract)
    BackgroundCellulartumorantigenp53,AntigenNY-CO-13,Phosphoproteinp53,Tumorsuppressorp53
    GeneID7157
    UniProtP04637
    ResearchAreaTranscriptionFactors
    Pathwaysp53Signaling,MAPKSignaling,PI3K-AktSignaling,Apoptosis,AMPKSignaling,ChromatinBinding,ER-NucleusSignaling,PositiveRegulationofEndopeptidaseActivity,HepatitisC,ProteintargetingtoNucleus,Autophagy
    SampleVolume100μL
    PlateUncoated
    Protocol
    1. Seed10,000-30,000cellsintoeachwellandincubateovernight.
    2. Applyvarioustreatment,inhibitorsoractivatorsaccordingtomanufacturesinstructions.
    3. Add100 μLofFixingSolutionintoeachwellandincubatefor20 minatRTwithshaking.
    4. Add200 μLofprepared1XQuenchingBufferandincubate20 minatRT.
    5. Add200 μLofBlockingSolutionandincubatefor1hat37 °C.
    6. Add50 μLof1Xanti-phospho-proteinspecificantibodyoranti-pan-proteinspecificantibodytoeachwellandincubatefor2hatRT.
    7. Add50 μLofprepared1XHRP-Anti-RabbitorMouseIgGandincubatefor1hatRT.
    8. Add100 μLofTMBOne-StepSubstrateReagenttoeachwell.
    9. Incubate30 minatRT.
    10. Add50 μLofStopSolutiontoeachwell.
    11. Readat450nmimmediately.
    AssayProcedure

    Seed10,000-30,000cellsintoeachwellandincubateovernight.Applyvarioustreatment,inhibitorsoractivatorsaccordingtomanufacturesinstructions.Add100 μLofFixingSolutionintoeachwellandincubatefor20 minatRTwithshaking.Add200 μLofprepared1XQuenchingBufferandincubate20 minatRT.Add200 μLofBlockingSolutionandincubatefor1hat37 °C.Add50 μLof1Xanti-phospho-proteinspecificantibodyoranti-pan-proteinspecificantibodytoeachwellandincubatefor2hatRT.Add50 μLofprepared1XHRP-Anti-RabbitorMouseIgGandincubatefor1hatRT.Add100 μLofTMBOne-StepSubstrateReagenttoeachwell.Incubate30 minatRT.Add50 μLofStopSolutiontoeachwell.Readat450nmimmediately.

    RestrictionsForResearchUseonly
    Storage-20°C
    StorageCommentTheentirekitmaybestoredat-20°Cforupto6monthsfromthedateofshipment.Avoidrepeatedfreeze-thawcycles.
    ExpiryDate6months

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