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AntibodiesOnline/Eukaryotic Translation Initiation Factor 1 (EIF1) (AA 1113), (full length) protei188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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AntibodiesOnline/Eukaryotic Translation Initiation Factor 1 (EIF1) (AA 1113), (full length) protei
ProteinName
EukaryoticTranslationInitiationFactor1(EIF1)
  • A121
  • EIF-1
  • EIF1A
  • ISO1
  • SUI1
  • Sui1-rs1
  • eukaryotictranslationinitiationfactor1
  • EIF1
  • Eif1
ProteinCharacteristics
AA1-113,fulllength
7AA1-113
5fulllength
4AA2-113
1AA1-79
Origin
Human
13Human
1Chicken
1Cow(Bovine)
1Mouse(Murine)
1Pongoabelii
Source
Escherichiacoli(E.coli)
9Escherichiacoli(E.coli)
3Yeast
1HEK-293Cells
1Wheatgerm
ProteinType
Recombinant
Purificationtag/Conjugate
GSTtag
Application
ELISA
Options
Bulkdiscount
Supplier
SupplierProductNo.
SequenceMSAIQNLHSFDPFADASKGDDLLPAGTEDYIHIRIQQRNGRKTLTTVQGIADDYDKKKLVKAFKKKFACNGTVIEHPEYGEVIQLQGDQRKNICQFLVEIGLAKDDQLKVHGF
CharacteristicsPleaseinquireifyouareinterestedinthisrecombinantproteinexpressedinE.coli,mammaliencellsorbybaculovirusinfection.Beawareaboutdifferencesinpriceandleadtime.
Purity90%
BackgroundNecessaryforscanningandinvolvedininitiationsiteselection.Promotestheassemblyof48SribosomalcomplexesattheauthenticinitiationcodonofaconventionalcappedmRNA.
MolecularWeight40.1kD
UniProtP41567
Comment

Theyeastproteinexpressionsystemisthemosteconomicalandefficienteukaryoticsystemforsecretionandintracellularexpression.Aproteinexpressedbythemammaliancellsystemisofveryhigh-qualityandclosetothenaturalprotein.Butthelowexpressionlevel,thehighcostofmediumandthecultureconditionsrestrictthepromotionofmammaliancellexpressionsystems.Theyeastproteinexpressionsystemserveasaeukaryoticsystemintegratetheadvantagesofthemammaliancellexpressionsystem.Aproteinexpressedbyyeastsystemcouldbemodificatedsuchasglycosylation,acylation,phosphorylationandsoontoensurethenativeproteinconformation.Itcanbeusedtoproduceproteinmaterialwithhighaddedvaluethatisveryclosetothenaturalprotein.Ourproteinsproducedbyyeastexpressionsystemhasbeenusedasrawmaterialsfordownstreampreparationofmonoclonalantibodies.

RestrictionsForResearchUseonly
FormatLyophilized
Concentration0.2-2mg/mL
BufferTris-basedbuffer,50%glycerol
HandlingAdviceRepeatedfreezingandthawingisnotrecommended.Storeworkingaliquotsat4°Cforuptooneweek
Storage-20°C
StorageCommentStoreat-20°Cforextendedstorage,conserveat-20°Cor-80°C
SupplierImages
SDS-PAGE (SDS) image for Eukaryotic Translation Initiation Factor 1 (EIF1) (AA 1-113), (full length) protein (GST tag) (ABIN1046988)EukaryoticTranslationInitiationFactor1(EIF1)(AA1-113),(fulllength)protein(...
BackgroundpublicationsMendell,Medghalchi,Lake,Noensie,Dietz:"NovelUpf2porthologuessuggestafunctionallinkbetweentranslationinitiationandnonsensesurveillancecomplexes."in:Molecularandcellularbiology,Vol.20,Issue23,pp.8944-57,2000(PubMed).

Sheikh,Fernandez-Salas,Yu,Hussain,Dinman,Peltz,Huang,Fornace:"Cloningandcharacterizationofahumangenotoxicandendoplasmicreticulumstress-induciblecDNAthatencodestranslationinitiationfactor1(eIF1(A121/SUI1))."in:TheJournalofbiologicalchemistry,Vol.274,Issue23,pp.16487-93,1999(PubMed).

Fields,Adams:"Expressedsequencetagsidentifyahumanisologofthesuiltranslationinitiationfactor."in:Biochemicalandbiophysicalresearchcommunications,Vol.198,Issue1,pp.288-91,1994(PubMed).


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