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AntibodiesOnline/Colony Stimulating Factor 2 (GranulocyteMacrophage) (CSF2) (Active) Protein/ABIN9188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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AntibodiesOnline/Colony Stimulating Factor 2 (GranulocyteMacrophage) (CSF2) (Active) Protein/ABIN9
ProteinName
ColonyStimulatingFactor2(Granulocyte-Macrophage)(CSF2)
  • CSF2
  • GM-CSF
  • GMCSF
  • CSF
  • Csfgm
  • Gm-CSf
  • MGI-IGM
  • Gm-csf
  • Gmcsf
  • granulocyte-macrophagecolony-stimulating-factor
  • colonystimulatingfactor2(granulocyte-macrophage)
  • granulocyte-macrophagecolony-stimulatingfactor
  • granulocyte-macrophagecolonystimulatingfactor
  • GM-CSF
  • CSF2
  • Csf2
Origin
Human
77Human
24Mouse(Murine)
14Rat(Rattus)
6RhesusMonkey
5Pig(Porcine)
4Dog(Canine)
2Cow(Bovine)
1Cat(Feline)
1Greenmonkey(Chlorocebus)
1GuineaPig
1Macaque
1Sheep(Ovine)
1Wildboar(Susscrofa)
Source
Escherichiacoli(E.coli)
65Escherichiacoli(E.coli)
9Yeast
8HumanCells
7Yeast(Pichiapastoris)
4Plant
3Human
3Sf-9cells
2CHO
2HEK-293
2HEK-293Cells
2MammalianCells
1Baculovirus/Sf9
1CHOCells
1InsectCells
1Nicotianabenthamiana
1Pichia
1Wheatgerm
ProteinType
Recombinant
BiologicalActivity
Active
Application
SDS-PAGE(SDS)
Options
Bulkdiscount
Supplier
SupplierProductNo.
SequenceMAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
CharacteristicsPurifiedrecombinantHumanGMCSFprotein
ExpressionSystem:E.coli
Bioactivity:TheED50forthiseffectis< 0.18 ng/mL. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
MolecularweightonSDS-PAGEwillappearhigher.
Purity>95%pure
EndotoxinLevel< 1.0 EU per µg of protein (determined by LAL method)
BackgroundHumanGM-CSFisa14.6kDaglobularproteinconsistingof128aminoacidcontainingtwodisulfidebonds.GM-CSFisahematopoieticgrowthfactorthatstimulatesthedevelopmentofneutrophilsandmacrophagesandpromotestheproliferationanddevelopmentofearlyerythroidmegakaryocyticandeosinophilicprogenitorcells.Itisproducedbyendothelialcells,monocytes,fibroblastsandT-lymphocytes.GM-CSFinhibitsneutrophilmigrationandenhancesthefunctionalactivityofthematureend-cells.RecombinanthumanGM-CSFwasexpressedinE.coliandpurifiedbyconventionalchromatography,afterrefoldingoftheisolatedinclusionbodiesinarenaturationbuffer.
AlternativeNames:MGC131935protein,GMCSFprotein,EosinophilColonyStimulatingFactorprotein,CMCSFprotein,Sargramostimprotein,GM-CSFprotein,PluripoietinAlphaprotein,GranulocyteMacrophageColonyStimulatingFactorprotein,Colony-stimulatingfactorprotein,,ColonyStimulatingFactor2protein,CSFprotein,Colonystimulatingfactorprotein,CSF2protein,GM-CSFprotein,CSF2protein,CSFAlphaprotein,Molgramostinprotein,Granulocyte-macrophagecolony-stimulatingfactorprotein,MGC138897protein,Granulocyte-macrophagecolonystimulatingfactorBurstPromotingActivityprotein
MolecularWeight14.6kDa(128AA)
ResearchAreaCytokines,GrowthFactors
PathwaysJAK-STATSignaling,CellularResponsetoMoleculeofBacterialOrigin
ApplicationNotesGM-CSFproteinhasbeenusedinSDSPAGEandmaybesuitableforuseinotherassaystobedeterminedbytheenduser.
AssayProcedure

Cellline:TF1(humanerythroleukemiccell).
MaintenanceCondition:10%FBSRPMI1640withGM-CSF
Assaymedium:10%FBSRPMI1640withoutGm-CSF
Celldensity:2x10,000cells/well(96wellplate,finalvolume100µL)
Starvation:24h,
AssaymediumIncubationtime:24hr(aftersampletreatment)
Concentrationrange:0.048ng/mL-25ng/mL
Detectionmethod:MTTassay

RestrictionsForResearchUseonly
FormatLiquid
Concentration1mg/mL
BufferSuppliedasaliquidinPBS,pH7.4.
HandlingAdviceAvoidrepeatedfreeze/thawcycles.
StorageRT/-20°C
StorageCommentStoreat4°Cforshorttermstorage(1/2weeks).Aliquotandstoreat-20°Cor-70°Cforlongtermstorage.
SupplierImages
SDS-PAGE (SDS) image for Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) Protein (ABIN934919)Figureannotationdenotesugofproteinloadedand%gelused.
Productcitedin:Spatz,Eibl,Hink,Wolf,Fischer,Mayr,Schernthaner,Eibl:"Impairedprimaryimmuneresponseintype-1diabetes.FunctionalimpairmentatthelevelofAPCsandT-cells."in:Cellularimmunology,Vol.221,Issue1,pp.15-26,2003(PubMed).


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