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AntibodiesOnline/CytoSelect™ 48well Cell Adhesion Assay (Fibrinogen, Colorimetric)/ABIN2344821/48 188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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AntibodiesOnline/CytoSelect™ 48well Cell Adhesion Assay (Fibrinogen, Colorimetric)/ABIN2344821/48

Reactivity
Human
Application
CellularAssay(CA)
Options
Bulkdiscount
Supplier
SupplierProductNo.
Independent Validation Badge
  • Successfullyvalidated(CellularAssay)
  • by FacultyofVeterinaryMedicine,UniversityofCalgary
  • No.#100062
  • Date10/06/2016
  •  
AntigenFibrinogen
MethodvalidatedCellularAssay
PositiveControlIshikawacells
NotesTheCellAdhesionAssay(Fibrinogen)ABIN2344821successfullydetectsfibrinogendependentcelladhesion.

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PrimaryAntibodyN/A
SecondaryAntibodyN/A
Protocol
  • IshikawacellsculturedinDMEM-F12mediumandgrownto80%confluency.
  • Cellsweretrypsinisedandre-suspendedinserum-freemedium.
  • AsubsetofcellswasthenincubatedwithRGDpeptidefor30min.
  • 0.5x106cellsofeachcellpopulationwereaddedtofibrinogencoatedwellsandBSAcontrolwellsandincubatedfor60min.
  • Theassaywasperformedaccordingtokit’smanual.
  • Measurement:
  • FG,RGD1FG,RGD2FG,no-RGD1FG,no-RGD2BSA
    0.0790.0790.1020.1040.034
    0.0750.0730.0990.1040.038
    0.0720.0710.1100.1300.029
    0.0660.0690.1050.105
    ExperimentalNotesFibrinogencoatedwellsresultedinhigherabsorbancereadingsthanBSAwell.CellsnotexposedtoRGDpeptidedisplayedsignificantlyhigherabsorbancevaluesthancellspreviouslyexposedtoRGDpeptide:thisconfirmsthatintegrinsexpressedonIshikawacellsmediateadhesiontofibrinogen.CellsexposedtoRGDpeptide(andaddedtofibrinogencoatedwells)displayedhigherabsorbancevaluesthancellsonBSAwells.ThisisconsistentwiththefactthatintegrinsinteractwithproteinsnotexclusivelyinaRGDdependentfashion.