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AntibodiesOnline/Citrate Buffer pH 6.0/ABIN1689348/125 mL188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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AntibodiesOnline/Citrate Buffer pH 6.0/ABIN1689348/125 mL
Application
Immunohistochemistry(IHC)
Options
Bulkdiscount
Supplier
SupplierProductNo.
CharacteristicsCitrateBuffer,afterdilution,isusedonformalin-fixed,paraffin-embeddedtissuesectionsmountedonglassslidesfortargetretrievalpriortoimmunohistochemistry(IHC)procedures.Whenperformedusingthisreagentandelevatedtemperature,targetretrievalcandisruptthecovalentbondsformedbyformalinintissue.Removalofthesebondscanallowrenaturationofproteinmoleculesandincreasedantibodyaccessibility,whichinturncanresultinincreasedantibodybinding(andthusincreasedstainingintensity)andimprovedsignaltonoiseratios.Thistypeofresultisobservedwithmanyprimaryantibodies.
Protocol1.Deparaffinizeandrehydratetissuesections.
2.DilutetheCitrateBuffer1:10withdeionizedwater.Example:20mlofconcentratedilutedin180mlofdeionizedwater.
3.ImmersesectionsincoplinjarfilledwiththedilutedCitrateBufferreagent.MicrowaveonHIGHpoweruntilboiling.
4.Keeptheslideswarmbyheatingfor10minutesonLOWpower.
5.Letthecoplinjarsitinmicrowaveforatleast20minutes.
6.Removeandrinsewithwater.
7.Rinsewithbufferandproceedwiththeappropriatestainingprotocolfortheprimaryantibodyinuse.
RestrictionsForResearchUseonly
FormatLiquid
Concentration10X
BufferCitrateBuffer,pH6.0.10xconcentrate.
PrecautionofUse1.Takereasonableprecautionswhenhandlingreagents.WearappropriatePersonalProtectiveEquipment.
2.Avoidcontactofreagentswitheyes,mucousmembranesandskin.Ifreagentscomeintocontactwithsensitiveareas,washwithcopiousamountsofwater.
3.Consultlocalorstateauthoritieswithregardtorecommendedmethodofdisposal.
4.Avoidmicrobialcontaminationofreagents.
StorageRT
StorageCommentTheusermustvalidateanyotherstorageconditions.Whenproperlystored,thereagentisstabletothedateindicatedonthelabel.Donotusethereagentbeyondtheexpirationdate.

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