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AntibodiesOnline/HEK293 Cells Immunoglobulin G2a (IgG2a) Isotype Control/ABIN2181277/200 μg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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AntibodiesOnline/HEK293 Cells Immunoglobulin G2a (IgG2a) Isotype Control/ABIN2181277/200 μg
Isotype
ImmunoglobulinG2a(IgG2a)
Reactivity
Mouse(Murine)
Host
HEK-293Cells
237Mouse
118Rat
BiologicalActivity
Active
Application
IsotypeControl(IsoC)
Options
Bulkdiscount
Supplier
SupplierProductNo.
IsotypeIgG2a
CharacteristicsThisproteincarriesno"tag".TheproteinhasacalculatedMWof26.4kDa.Asaresultofglycosylation,theproteinmigratesas25-30kDaunderreducing(R)condition,and50-60kDaundernon-reducing(NR)condition(SDS-PAGE).
Purity>95 %asdeterminedbySDS-PAGE.
Sterility0.22μmfiltered
EndotoxinLevelLessthan1.0EUperμgbytheLALmethod.
AlternativeNameIgG2a
BackgroundImmunoglobulinG2(IgG2)isamemberofmanyimmunoglobulinGdevelopedandsecretedbyeffectiveBcells.Inwakeofcuttingbypepsin,IgGisdividedintotwoF(ab)swithoneantigenbindingsiteandahighconservedFcsegment.TheFcsegmentbearsahighlyconservedN-glycosylationsite.TherearetwomembersofIgG2:IgG2aandIgG2b.ItwasfoundthatIgG2awassuperiortoIgG1inactivatingcomplement.Theglycosylationofthecirculatingimmunoglobulin-γ(IgG)antibodymoleculeschangesinrheumatoidarthritis.
MolecularWeight26.4kDa
UniProtP01863
ResearchAreaSecondaryAntibodies
ApplicationNotesOptimalworkingdilutionshouldbedeterminedbytheinvestigator.
RestrictionsForResearchUseonly
FormatLyophilized
ReconstitutionPleaseseeCertificateofAnalysisforspecificinstructions.Forbestperformance,westronglyrecommendyoutofollowthereconstitutionprotocolprovidedintheCoA.
Buffer50mMTris,100mMGlycine, pH7.5
HandlingAdviceAvoidrepeatedfreeze-thawcycles.
Storage-20°C
StorageCommentNoactivitylosswasobservedafterstorageat:Inlyophilizedstatefor1year(4°C),Afterreconstitutionundersterileconditionsfor3months(-70°C).
SupplierImages
SDS-PAGE (SDS) image for HEK-293 Cells Immunoglobulin G2a (IgG2a) Isotype Control (ABIN2181277)MouseIgG2aFc,TagFreeonSDS-PAGEunderreducing(R)andno-reducing(NR)conditio...
BackgroundpublicationsSikorav,Auffray,Rougeon:"Structureoftheconstantand3untranslatedregionsofthemurineBalb/cgamma2aheavychainmessengerRNA."in:Nucleicacidsresearch,Vol.8,Issue14,pp.3143-55,1981(PubMed).

Ollo,Auffray,Morchamps,Rougeon:"Comparisonofmouseimmunoglobulingamma2aandgamma2bchaingenessuggeststhatexonscanbeexchangedbetweengenesinamultigenicfamily."in:ProceedingsoftheNationalAcademyofSciencesoftheUnitedStatesofAmerica,Vol.78,Issue4,pp.2442-6,1981(PubMed).


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