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AntibodiesOnline/ORAI Calcium ReleaseActivated Calcium Modulator 1 (ORAI1) (AA 1304) protein (rho188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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AntibodiesOnline/ORAI Calcium ReleaseActivated Calcium Modulator 1 (ORAI1) (AA 1304) protein (rho
ProteinName
ORAICalciumRelease-ActivatedCalciumModulator1(ORAI1)
  • im:7146264
  • orai1
  • tmem142a
  • zgc:109721
  • orai2
  • ORAI1
  • LOC100218031
  • CRACM1
  • ORAT1
  • TMEM142A
  • D730049H07Rik
  • Tmem142a
  • orai-1
  • RGD1311873
  • tmem142b
  • ORAIcalciumrelease-activatedcalciummodulator1b
  • ORAIcalciumrelease-activatedcalciummodulator1
  • ORAIcalciumrelease-activatedcalciummodulator2
  • orai1b
  • orai1
  • ORAI1
  • Orai1
  • LOC100218031
  • orai2
ProteinCharacteristics
AA1-304
1AA1-301
Origin
Mouse(Murine)
1Human
Source
InsectCells
1InsectCells
ProteinType
Recombinant
Purificationtag/Conjugate
ThisORAI1proteinislabelledwithrho-1D4tag.
Application
Crystallization(Crys),ELISA,SDS-PAGE(SDS),WesternBlotting(WB)
Options
Bulkdiscount
Supplier
SupplierProductNo.
SequenceMHPEPAPPPSHSNPELPVSGGSSTSGSRRSRRRSGDGEPSGAPPLPPPPPAVSYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDTDHDYPPGLLIVFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKRQAGQPSPTKPPAESVIVANHSDSSGITPGEAAAIASTAIMVPCGLVFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHSLTPGTHYA
Sequencewithouttag.Taglocationisatthediscretionofthemanufacturer.Ifyouhaveaspecialrequest,pleasecontactus.
Characteristics
  • MadeinGermany-fromdesigntoproduction-byhighlyexperiencedproteinexperts.
  • MouseOrai1Protein(raisedinInsectCells)purifiedbymulti-step,protein-specificprocesstoensurecrystallizationgrade.
  • State-of-the-artalgorithmusedforplasmiddesign(Genesynthesis).
PurificationThreesteppurificationofmembraneproteinsexpressedinbaculovirusinfectedSF9insectcells:
  1. Membraneproteinsarefractionedbyultracentrifugationandsubsequentlysolubilizedwithdifferentdetergents(detergentscreen).SamplesareanalyzedbyWesternblot.
  2. Thebestperformingdetergentisusedforsolubilizationandtheproteinsarepurifiedviatheirrho1D4tagviatworho1D4antibodycolumns:oneDTTresistant,theotheronenot.EluatefractionsareanalyzedbyWesternblot.
  3. Proteincontainingfractionsofthebestpurificationaresubjectedtosecondpurificationstepthroughsizeexclusionchromatograph.EluatefractionsareanalyzedbySDS-PAGEandWesternblot.
Purity>95 %asdeterminedbySDSPAGE,SizeExclusionChromatographyandWesternBlot.
Sterility0.22μmfiltered
EndotoxinLevelProteinisendotoxin-free.
GradeCrystallographygrade
BackgroundCa(2+)release-activatedCa(2+)(CRAC)channelsubunitwhichmediatesCa(2+)influxfollowingdepletionofintracellularCa(2+)storesandchannelactivationbytheCa(2+)sensor,STIM1.CRACchannelsarethemainpathwayforCa(2+)influxinT-cellsandpromotetheimmuneresponsetopathogensbyactivatingthetranscriptionfactorNFAT.{ECO:0000250|UniProtKB:Q96D31}.
MolecularWeight34.2kDaIncludingtag.
UniProtQ8BWG9
ResearchAreaAdaptiveImmunity,Signaling
PathwaysTCRSignaling
ApplicationNotesInadditiontotheapplicationslistedaboveweexpecttheproteintoworkforfunctionalstudiesaswell.Astheproteinhasnotbeentestedforfunctionalstudiesyetwecannotofferaguranteethough.
Comment

Proteinhasnotbeentestedforactivityyet.Incasesinwhichitishighlylikelythattherecombinantproteinwiththedefaulttagwillbeinsolubleourproteinlabmaysuggestahighermolecularweighttag(e.g.GST-tag)insteadtoincreasesolubility.Wewilldiscussallpossibleoptionswithyouindetailtoassurethatyoureceiveyourproteinofinterest.

RestrictionsForResearchUseonly
FormatLiquid
Buffer100mMNaCL,20mMHepes,10%glycerol.pHvalueisatthediscretionofthemanufacturer.
HandlingAdviceAvoidrepeatedfreeze-thawcycles.
Storage-80°C
StorageCommentStoreat-80°C.
ExpiryDateUnlimited(ifstoredproperly)
SupplierImages
image for ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 1-304) protein (rho-1D4 tag) (ABIN3125369)„CrystallographyGrade“proteinduetomulti-step,protein-specificpurificationprocess

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