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Bpsbioscience/ACK1, GSTtag/40400/10 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Bpsbioscience/ACK1, GSTtag/40400/10 µg
Species:Human
HostSpecies:Sf9insectcells
MW:68kDa
Genbank#:NM_005781
Tag(s):N-terminalGST-tag
a.a:110–476(end)
Description:
HumanAck1(GenBankAccessionNo.NM_005781),a.a.110-476(end)withN-terminalGST-tag,MW=68kDa,expressedinSf9insectcellsviaabaculovirusexpressionsystem.
Synonym(s):
ACK1,activatedCdc42-associatedkinase1
AssayConditions:
AssaywasdoneinKinasebuffercontaining0.2mMDTTusingPoly-(Glu4:Tyr)-biotinsubstrate(0.2mg/ml)and20mMATP.Reactionwasdoneat30°Cfor30minutes.AmountofATPtransferredwascalculatedusingKinase-Gloreagent(Promega).
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and16mMglutathione
Format:
Aqueousbuffersolution
Storage/Stability:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Eley,L.etal.,Biochem.Biophys.Res.Commun.371(4),877-882(2008).
2.Grovdal,L.M.etal.,Exp.CellRes.314(6),1292-1300(2008).
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/CytoplasmicTyr
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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