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Bpsbioscience/EGFR (L858R), GSTtag/40189/10 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Bpsbioscience/EGFR (L858R), GSTtag/40189/10 µg
Species:Human
HostSpecies:Sf9insectcells
MW:88kDa
Genbank#:NM_005228
Tag(s):N-terminalGST-tag
a.a:668–1210
Description:
HumanEGFRL858R(GenBankAccessionNo.NM_005228),a.a.668-1210withsubstitutionofLeucine858toArginineandanN-terminalGST-tag,MW=88kDa,expressedinabaculovirusexpressionsystem.
UniProt
Q15822P00533
Synonym(s):
EGFR,EpidermalGrowthFactorReceptor,ERBB,ErbB-1,HER1,MENA
SpecificActivity:
340pmol/min/μg.
AssayConditions:
Assaywasperformedinakinasebuffercontaining0.2mMDTTusingPoly-(Glu4:Tyr)-biotinsubstrate(0.2mg/ml)and20μMATP.Reactionwasdoneat30°Cfor30min.ATPtransferredwascalculatedusingKinase-Gloreagent(Promega).
Formulation:
40mMTris-HClpH8.0,110mMNaCl,2.2mMKCl,0.04%Tween-20,20%glyceroland3mMDTT.
Format:
Aqueousbuffersolution
Storage/Stability:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Adachi,S.etal.,CancerRes.67(13),6493-6501(2007)
2.Rosano,L.etal.,CancerRes.67(13),6351-6359(2007).

ApplicationReference:
Figueroa,M.,etal.,J.Antibiotics.65,559-564(2012).
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/ReceptorTyrosine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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