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2,5-dihydroxybenzoic acid (DHB) is a commonly used MALDI mass spectrometry (MALDI-MS) matrix with λmax at 337 nm and 355 nm. DHB is a good MALDI matrix for general MALDI-MS of protein digests, carbohydrates, oligosacharides, glycopeptides and both proteins and peptides below 10 kDa. DHB matrix is also well suited for the negative ion MALDI-MS glycolipids.
