Avertin (2, 2, 2Tribromoethanol)

This is prepared as a 1.2% solution and used in mice at a dosage rate of 0.2 ml/10grams body weight which approximates 240 mg/kg. This compound is subject to photodegradation and should be stored at 4°C in the dark, or prepared fresh before use.• Avertin Stock solution:25 grams avertin (2, 2, 2-Tribromoethanol) [Sigma-Aldrich, #T4,840-2]15. 5 ml tert-Amyl Alcohol (2-methyl-2-butanol) [Fisher, #A730-1]Mix ~12 hours in a dark bottle at room temperature. [1600 mg/ml]• Working solution:0.5 ml Avertin stock39.5 ml 0.9% saline (NaCl)Mix in dark container. Filter sterilize and store at 4°C. [20 mg/ml]• Dosages:Embryo transfers 0.45 to 0.55 ml IPBiopsies/Milking 0.5 to 1.0 ml IPSacrifices ~1.0 ml IP•It will take the animal ~5 minutes to become fully anesthetized.An additional 0.1 to 0.2 ml of Avertin can be given as necessary.The animal will remain anesthetized for ~20 minutes.• References:Papaioannou VE, Fox JG. Efficacy of tribromoethanol anesthesia in mice.Lab Anim Sci1993 Apr;43(2):189-92Reid WC, Carmichael KP, Srinivas S, Bryant JL. Pathologic changes associated withuse of tribromoethanol (avertin) in the Sprague Dawley rat. Lab Anim Sci 1999Dec;49(6):665-7Zeller W, Meier G, Burki K, Panoussis B Adverse effects of tribromoethanol as used inthe production of transgenic mice.Lab Anim 1998 Oct;32(4):407-13