PCRboost(R) PCR增强试剂

产品指标

形式	150 ?L, 1 mL, 10 mL
保存期	3个月
保存温度	15 - 25 °C
下游应用	质粒纯化，细菌繁殖，甘油保存制备

Genomic DNA (gDNA) from human blood was purified using the QIAamp? DNA Blood Mini Kit., and 500ng was exposed to 70°C for 20h. 1 ?l (~50ng) of thermally degraded DNA or control DNA stored at 4°C was used as template for PCR reactions. The reactions were set up using 2.5 U Taq DNA polymerase (NEB), 3μl 10x thermopol reaction buffer (NEB), 0.5?l dNTPs (10?M each nucleotide), 0.5?l each of human β-actin forward and reverse primers. The volume in the thermally degraded DNA PCR reaction tubes was brought up either in water or in PCRboost. Following PCR amplification, 10?l of each PCR reaction was run on an agarose gel. A) 500ng human genomic DNA stored at 4°C and thermally degraded at 70°C for 20h run on an agarose gel. L: 1kb ladder. B) 50ng of cold-stored (4°C) and thermally degraded DNA used as template in PCR reactions amplifying the human β-actin gene. PCR reactions were brought up to volume either in water or PCRboost. L: ladder.