Innova Biosciences/High Throughput Colorimetric GTPase Assays (inc. PiColorLock)/6020120/192 assays

Thisnonradioactivecolorimetricassaykitcontainsallthenecessaryreagentsformeasuringenzymeactivity(everythingincludedinthePiColorlock™reagentandmore)andisidealforhighthroughputdrugscreening.

ThekitscontainPiColorlock™,anon-radioactive,superiorphosphatedetectionreagent.

KeyfeaturesofPiColorLock™include:

ColorimetricAssay–competitorassaysareradioactive
Specialadditiveensureslowbackgroundswithacid-labilesubstrates
Unparalleledstabilityofphosphate-dyecomplexes
Reagentiscompatiblewithalmostanyassaybuffer
Noinhibitionofcolordevelopmentbyhighconcentrationsofprotein
Stablereagentformulation–longshelflife

ColorimetricassaysforGTPasesareinvariablybasedontheformationofcoloredcomplexesbetweenaninorganicphosphateandadyemoleculeunderacidicconditions.

Competitorassaysarebesetwithseveralproblemsincludingreagentprecipitation.ThehighstabilityofPiColorLock™ensureshighstabilityofthecoloreddye-phosphatecomplexes(greencolor-seebelow).

KitComponents:

Componentsinthe2-plateGTPassaykit(5-platekitamountsinbrackets)

Storeat4°C:

1x10mlofPiColorLock™*(1x25ml)
1x0.25mlofAccelerator(1x0.5ml)
1x5mlofStabiliser(1x10ml)
1x1.5mlof0.1MMgCl2(2x1.5ml)
1x5mlof0.5MTrispH7.5(1x10ml)
1x5mlof0.1mMPistandard(1x10ml)

*Takecare,thesolutionisveryacidic

Storeat-20or-70^oC:

4vialsoflyophilizedGTP(10vials)
Storeatroomtemperature
2x96-wellplates(5plates)

ThedatabelowisforthePiColorLock™reagentwhichisincludedinGTPaseassaykits.

Figure1.OurPiColorLockhasalsobeendesignedtohavealargelinearrange,thusreducingtheneedforsampledilution.Competitors’productsarelinearoveramuchnarrowerrangeofconcentrations.

TheRiceDynamin-RelatedProteinOsDRP1ENegativelyRegulatesProgrammedCellDeathbyControllingtheReleaseofCytochromecfromMitochondria-PLoSOne(2017)

Viewreference

G2385RandI2020TMutationsIncreaseLRRK2GTPaseActivity - BioMedResearchInternational(2016)

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Comparativereal-timePCRandenzymeanalysisofselectedgender-associatedmoleculesinSchistosomajaponicum-Parasitology(2008)

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CharacterizationofHumanGTPBP3,aGTP-BindingProteinInvolvedinMitochondrialtRNAModification-MolecularandCellularBiology(2008)

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GProtein-CoupledReceptor-PromotedTraffickingofG?1?2LeadstoAKTActivationatEndosomesviaaMechanismMediatedbyG?1?2-Rab11aInteraction–MolecularBiologyoftheCell(2008)

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StructureofERAincomplexwiththe3?endof16SrRNA:Implicationsforribosomebiogenesis-PNAS(2009)

Viewreference

High-levelproductionandcharacterizationofaG-proteincoupledreceptorsignalingcomplex-FEBSJournal(2009)

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Speckled-likePatternintheGerminalCenter(SLIP-GC),aNuclearGTPaseExpressedinActivation-inducedDeaminase-expressingLymphomasandGerminalCenterBCells-JBC(2009)

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IdentificationandCharacterizationofaLeucine-RichRepeatKinase2(LRRK2)ConsensusPhosphorylationMotif-PLoSOne(2010)

Viewreference

AIP1FunctionsasArf6-GAPtoNegativelyRegulateTLR4Signaling-JBC(2010)

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RoleoftheG-proteinandtyrosinekinase—Rho/ROKpathwaysin15-hydroxyeicosatetraenoicacidinducedpulmonaryvasoconstrictioninhypoxicrats-JBiochem(2010)

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GTPaseactivityplaysakeyroleinthepathobiologyofLRRK2-PLoSGenetics(2010)

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AIP1FunctionsasArf6-GAPtoNegativelyRegulateTLR4Signaling-JBC(2010)

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GTPaseActivityandNeuronalToxicityofParkinsonsDisease–AssociatedLRRK2IsRegulatedbyArfGAP1-PLoSGenetics(2012)

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ArfGAP1IsaGTPaseActivatingProteinforLRRK2:ReciprocalRegulationofArfGAP1byLRRK2-JournalofNeuroscience(2012)

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Leucyl-tRNASynthetaseIsanIntracellularLeucineSensorforthemTORC1-SignalingPathway-Cell(2012)

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TheMitochondrialPhosphatasePGAM5FunctionsattheConvergencePointofMultipleNecroticDeathPathways-Cell(2012)

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TubulinnucleotidestatuscontrolsSas-4-dependentpericentriolarmaterialrecruitment-NatureCellBiology(2012)

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Theselenocysteine-specificelongationfactorcontainsanovelandmulti-functionaldomain-TheJournalofBiologicalChemistry(2012)

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PhosphorylationandnitrationoftyrosineresiduesaffectfunctionalpropertiesofSynaptophysinandDynaminI,twoproteinsinvolvedinexo-endocytosisofsynapticvesicles-BBAMolecularCellResearch(2012)

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DefectiveAxonalTransportofRab7GTPaseResultsinDysregulatedTrophicSignaling-TheJournalofNeuroscience(2013)

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ThromboxaneReceptorHyper-ResponsivenessinHypoxicPulmonaryHypertensionRequiresSerine324-BritishJournalofPharmacology(2013)

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ToxoplasmaGRA7effectorincreasesturnoverofimmunity-relatedGTPasesandcontributestoacutevirulenceinthemouse-PNAS(2013)

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ThePIKEHomologCentauringammaRegulatesDevelopmentalTiminginDrosophila-PLoSOne(2014)

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InteractionoftheArabidopsisGTPaseRabA4cwithItsEffectorPMR4ResultsinCompletePenetrationResistancetoPowderyMildew-PlantCell(2014)

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BlockageofGSK3?-mediatedDrp1phosphorylationprovidesneuroprotectioninneuronalandmousemodelsofAlzheimer’sdisease-NeurobiologyofAging(2014)

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ARac1GTPaseisacriticalfactorintheimmuneresponseofshrimp(Litopenaeusvannamei)toVibrioalginolyticusinfection-DevelopmentalandComparativeImmunology(2015)

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RGS19convertsirondeprivationstressintoagrowth-inhibitorysignal-BiophysicalandBiochemicalResearchCommunications(2015)

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Innova Biosciences/High Throughput Colorimetric GTPase Assays (inc. PiColorLock)/6020120/192 assays

KeyfeaturesofPiColorLock™include:

KitComponents:

TheRiceDynamin-RelatedProteinOsDRP1ENegativelyRegulatesProgrammedCellDeathbyControllingtheReleaseofCytochromecfromMitochondria-PLoSOne(2017)

G2385RandI2020TMutationsIncreaseLRRK2GTPaseActivity - BioMedResearchInternational(2016)

Comparativereal-timePCRandenzymeanalysisofselectedgender-associatedmoleculesinSchistosomajaponicum-Parasitology(2008)

CharacterizationofHumanGTPBP3,aGTP-BindingProteinInvolvedinMitochondrialtRNAModification-MolecularandCellularBiology(2008)

GProtein-CoupledReceptor-PromotedTraffickingofG?1?2LeadstoAKTActivationatEndosomesviaaMechanismMediatedbyG?1?2-Rab11aInteraction–MolecularBiologyoftheCell(2008)

StructureofERAincomplexwiththe3?endof16SrRNA:Implicationsforribosomebiogenesis-PNAS(2009)

High-levelproductionandcharacterizationofaG-proteincoupledreceptorsignalingcomplex-FEBSJournal(2009)

Speckled-likePatternintheGerminalCenter(SLIP-GC),aNuclearGTPaseExpressedinActivation-inducedDeaminase-expressingLymphomasandGerminalCenterBCells-JBC(2009)

IdentificationandCharacterizationofaLeucine-RichRepeatKinase2(LRRK2)ConsensusPhosphorylationMotif-PLoSOne(2010)

AIP1FunctionsasArf6-GAPtoNegativelyRegulateTLR4Signaling-JBC(2010)

RoleoftheG-proteinandtyrosinekinase—Rho/ROKpathwaysin15-hydroxyeicosatetraenoicacidinducedpulmonaryvasoconstrictioninhypoxicrats-JBiochem(2010)

GTPaseactivityplaysakeyroleinthepathobiologyofLRRK2-PLoSGenetics(2010)

AIP1FunctionsasArf6-GAPtoNegativelyRegulateTLR4Signaling-JBC(2010)

GTPaseActivityandNeuronalToxicityofParkinsonsDisease–AssociatedLRRK2IsRegulatedbyArfGAP1-PLoSGenetics(2012)

ArfGAP1IsaGTPaseActivatingProteinforLRRK2:ReciprocalRegulationofArfGAP1byLRRK2-JournalofNeuroscience(2012)

Leucyl-tRNASynthetaseIsanIntracellularLeucineSensorforthemTORC1-SignalingPathway-Cell(2012)

TheMitochondrialPhosphatasePGAM5FunctionsattheConvergencePointofMultipleNecroticDeathPathways-Cell(2012)

TubulinnucleotidestatuscontrolsSas-4-dependentpericentriolarmaterialrecruitment-NatureCellBiology(2012)

Theselenocysteine-specificelongationfactorcontainsanovelandmulti-functionaldomain-TheJournalofBiologicalChemistry(2012)

PhosphorylationandnitrationoftyrosineresiduesaffectfunctionalpropertiesofSynaptophysinandDynaminI,twoproteinsinvolvedinexo-endocytosisofsynapticvesicles-BBAMolecularCellResearch(2012)

DefectiveAxonalTransportofRab7GTPaseResultsinDysregulatedTrophicSignaling-TheJournalofNeuroscience(2013)

ThromboxaneReceptorHyper-ResponsivenessinHypoxicPulmonaryHypertensionRequiresSerine324-BritishJournalofPharmacology(2013)

ToxoplasmaGRA7effectorincreasesturnoverofimmunity-relatedGTPasesandcontributestoacutevirulenceinthemouse-PNAS(2013)

ThePIKEHomologCentauringammaRegulatesDevelopmentalTiminginDrosophila-PLoSOne(2014)

InteractionoftheArabidopsisGTPaseRabA4cwithItsEffectorPMR4ResultsinCompletePenetrationResistancetoPowderyMildew-PlantCell(2014)

BlockageofGSK3?-mediatedDrp1phosphorylationprovidesneuroprotectioninneuronalandmousemodelsofAlzheimer’sdisease-NeurobiologyofAging(2014)

ARac1GTPaseisacriticalfactorintheimmuneresponseofshrimp(Litopenaeusvannamei)toVibrioalginolyticusinfection-DevelopmentalandComparativeImmunology(2015)

RGS19convertsirondeprivationstressintoagrowth-inhibitorysignal-BiophysicalandBiochemicalResearchCommunications(2015)