TheTruSeqStrandedTotalLibraryPreparationkitprovidesaclearviewofthetranscriptomewithastreamlined,cost-efficient,andscalablesolutionfortotalRNAanalysiswithsequencing.
Compatiblewithawiderangeofsamples,includinglow-qualityandFFPE,TruSeqStrandedTotalRNAkitscoupleallofthebenefitsofTruSeqRNApreparationkitswithRibo-ZeroribosomalRNAreductionchemistry,providinganalysisofcodingandmultipleformsofnoncodingRNAwithprecisemeasurementofstrandorientation,uniformcoverage,andhigh-confidencediscoveryoffeaturessuchasalternativetranscripts,genefusions,andallele-specificexpression.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatiblewiththislibrarypreparationkit
TheTruSeqStrandedTotalRNALibraryPrepkitisavailableinmultipleversionstofitspecificstudydesignneeds:
TheTruSeqStrandedTotalLibraryPreparationkitisdesignedforhuman,mouseandratsequences,butmaybeapplicabletoavarietyofeukaryoticspecies.*
*Forinformationonaparticularspeciesofinterest,contactIlluminaTechnicalSupport.
| InputQuantity | 0.1–1ughigh-qualitytotalRNA.Lower-qualitysamplesmayrequirefurtheroptimization. |
| ContentSpecifications | CapturescodingRNAplusmultipleformsofnon-codingRNA |
| MechanismofAction | Bead-basedrRNAdepletion,cDNAsynthesis,andPCR |
| Multiplexing | Low-throughputkits:Poolupto12samples.Orpoolupto24sampleswithsetsAandBtogether,High-throughputkits:Prepare96uniquelyindexedsamples. |
| SystemCompatibility | NovaSeq5000,GenomeAnalyzerIIx,HiSeq2000,NextSeq550,HiSeq3000,HiSeq1000,HiSeq1500,NextSeq500,HiSeq2500,NovaSeq6000,HiSeq4000 |
| VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,NovelTranscripts,TranscriptVariants |
| SpecializedSampleTypes | FFPE |
| Method | Whole-TranscriptomeSequencing |
| SpeciesCategory | Mouse,Human,Rat |
| Technology | Sequencing |
| AutomationCapability | LiquidHandlingRobots |