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TheB8.2C12monoclonalantibodyreactswithmouseTIM-3(Tcellimmunoglobulinandmucindomain-3)alsoknownasCD366.ThisantibodybindstotheBALB/calleleofTIM-3whilereactivitytotheC57Bl/6alleleissignificantlyweaker.TIM-3isa60kDamemberoftheTIMfamilyofimmunecheckpointreceptorsandexistsasatypeItransmembraneglycoproteinwithamucin-likedomaininitsextracellularportionandatyrosinephosphorylationmotifinitscytoplasmicportion.TIM-3isspecificallyexpressedathighlevelsonthesurfaceofTh1lymphocyteswhereasTh2lymphocytesexpressTIM-1andTIM-2.TIM-3activationoccursviabindingtothecell-associatedC-typelectingalectin-9.UponbindingTIM-3inducesapoptosisofTh1cells.InhibitionofTIM-3signalinginmicehasbeenshowntoexacerbateexperimentalautoimmuneencephalomyelitis,promoteIFNγproductionandTh1cellproliferation.Tim-3hasalsobeenshowntoberequiredfortheinductionoftolerance,asbothTIM-3knockoutanimalsandmicetreatedwithTIM-3-Igfusionproteindisplaydefectsintheinductionofantigen-specifictolerance.Additionally,TIM-3signalingiscurrentlybeingexploredasacancerimmunotherapytargetasCD8TcellswhichexpressbothTIM-3andPD-1exhibitgreaterdefectsinbothcell-cycleprogressionandeffectorcytokineproductionthancellsthatexpressPD-1alone.
| Isotype | RatIgG1 |
| RecomendedIsotypeControl(s) | InVivoMAbratIgG1isotypecontrol,anti-horseradishperoxidase InVivoMAbratIgG1Isotypecontrol,anti-trinitrophenol |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
| Immunogen | MouseTim-3protein/Freundadjuvant |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Undilutedat4°Cinthedark |