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Biohelix/Thermostable Helicase/H0240S/20 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品

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Biohelix/Thermostable Helicase/H0240S/20 µg

Description:

ThermostableDNAHelicaseisaDNAhelicasethatusestheenergygeneratedbythehydrolysisofnucleosidetriphosphatestobreakthehydrogenbondslinkingthetwostrandstogetherinduplexDNA.Itisactiveinatemperaturerangeof45to65ºC.

SUPPLIEDWITH:
10XAnnealingBufferII
100mMMgSO₄

SuppliedbyUsers:ATP

Source:

PurifiedfromanE.colistrainthatoverexpressesthehelicasegeneisolatedfromathermophilicmicroorganism.

Reactioncondition:

1XAnnealingBufferII:
10mMKCl
20mMTris-HCl,pH8.8@25°C

Supplementwith4mMMgSO₄and3mMATP.
Incubateat45-65ºC.

StorageConditions:

StorageTemperature:
-20ºC

Concentration:
0.5mg/ml

StorageBuffer:
20mMTris-HClpH7.4
200mMNaCl
1mMDTT
0.1mMEDTA
50%Glycerol

QualityControl:

QualityAssuranceStatement:
ThermostableHelicaseispurifiedfreeofcontaminatingendonucleasesandexonucleases.EachlotistestedforDNAunwindingactivity.

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