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Bpsbioscience/Abl Kinase, Histag/40411/10 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Bpsbioscience/Abl Kinase, Histag/40411/10 µg
Species:Human
HostSpecies:Sf9insectcells
MW:33kDa
Genbank#:NM_00731
Tag(s):N-terminalHis-tag
a.a:248–531
Description:
HumanAbelsonmurineleukemiaviraloncogenehomolog1(Abl-1)(GenBankAccessionNo.NM_007313),a.a.248-531,withN-terminalHis-tag,MW=33kDa,expressedinaBaculovirus-infectedSf9cellexpressionsystem
UniProt
P00519
Synonym(s):
ABL1,ABL-1,Abelsonmurineleukemiaviraloncogenehomolog1,JTK7
SpecificActivity:
2,700pmoles/min/μg.
AssayConditions:
AssaywasperformedinKinasebuffercontaining0.2mMDTTusingAbltidesubstrate(0.2mg/ml)and20mMATP.Reactionwasdoneat30°Cfor30min.AmountofATPtransferredwascalculatedusingKinase-Glo®reagent(Promega).
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween-20,3mMDTT,200mMimidazole,and20%glycerol.
Format:
Aqueousbuffersolution
Storage/Stability:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):

1.Cao,C.,etal.2003.J.Biol.Chem.278(15):12961–7.
2.Yano,H.,etal.2000.J.Neurosci.Res.59(3):356–364.

ApplicationReference:
LASP1isanovelBCR-ABLsubstrateandaphosphorylation-dependentbindingpartnerofCRKLinchronicmyeloidleukemia(2014)

Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/ReceptorTyrosine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.

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