Adipogen/ZVADFMK (Cell permeable)/AGCP30002M001/1 mg

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ProductDetails
Synonyms	Z-VAD(OMe)-FMK;Z-Val-Ala-DL-Asp(OMe)-FMK;pan-CaspaseInhibitor
ProductType	Chemical
Properties
Formula	C₂₂H₃₀FN₃O₇
MW	467.5
Sequence	Z-Val-Ala-DL-Asp(OMe)-fluoromethylketone
CAS	187389-52-2and634911-81-2
PurityChemicals	≥95%(HPLC)
Appearance	Whitesolid.
Solubility	SolubleinDMSO,acetonitrileordimethylformamide.
OtherProductData	Preparea10mMstocksolutionofthecaspaseinhibitorinhighqualityDMSO.Add2μlofstocksolutionto1mlofculturemediumcontainingcellstogive20μMfinalconcentration.Effectivefinalconcentrationsareestimatedtobe5-20μM.Ifaparticularinhibitorisappliedathigherconcentrations,specificityforanindividualcaspaseorevenforthecaspasefamily(>100μM)mightbecompromised.DMSOconcentrationsabove0.2%maycausecellulartoxicity,thusmaskingtheeffectofthecaspaseinhibitor.Forinvivoexperimentsextending12to48hours,freshinhibitormayhavetobeadded(injected)duetoinactivationbyreactionwithcysteineprotease.
InChiKey	MIFGOLAMNLSLGH-SXUUOERCBK
ShippingandHandling
Shipping	AMBIENT
ShortTermStorage	+4°C
LongTermStorage	-20°C
HandlingAdvice	Keepcoolanddry.
Use/Stability	Stableforatleast3yearsafterreceiptwhenstoredat-20°C.
Documents
MSDS	DownloadPDF
ProductSpecificationSheet
Datasheet	DownloadPDF

Cellpermeable,non-selectivebroad-spectrumcaspaseinhibitor[1,3,7,8].Bindsirreversiblytothecatalyticsiteofcaspaseproteases[1].
ThepeptideisO-methylatedintheP1positiononasparticacid,providingenhancedstabilityandincreasedcellpermeability[1].
InhibitsICE-familyprotease/caspaseprocessing,leadingtoapoptosisandautophagyinduction[2-4,6,9].
Decreasesproteasomeactivity[5].
Potentinhibitorofcaspase-1activationinNLRP3/NALP3-inducedcells[10].
Usedinapoptosisandinflammasomestudies.

ProductReferences

Benzyloxycarbonyl-Val-Ala-Asp(OMe)fluoromethylketone(Z-VAD.FMK)inhibitsapoptosisbyblockingtheprocessingofCPP32:E.A.Slee,etal.;Biochem.J.315,21(1996)
Differentinterleukin-1betaconvertingenzyme(ICE)familyproteaserequirementsfortheapoptoticdeathofTlymphocytestriggeredbydiversestimuli:A.Sarin,etal.;J.Exp.Med.184,2445(1996)
Processing/activationofatleastfourinterleukin-1betaconvertingenzyme-likeproteasesoccursduringtheexecutionphaseofapoptosisinhumanmonocytictumorcells:M.MacFarlane,etal.;J.CellBiol.137,469(1997)
Processing/activationofcaspases,-3and-7and-8butnotcaspase-2,intheinductionofapoptosisinB-chroniclymphocyticleukemiacells:D.King,etal.;Leukemia12,1553(1998)
Proteasomeactivitiesdecreaseduringdexamethasone-inducedapoptosisofthymocytes:J.Beyette,etal.;Biochem.J.332,315(1998)
JNK(c-JunN-terminalkinase)andp38activationinreceptor-mediatedandchemically-inducedapoptosisofT-cells:differentialrequirementsforcaspaseactivation:M.MacFarlane,etal.;Biochem.J.348,93(2000)
Statin-inducedproinflammatoryresponseinmitogen-activatedperipheralbloodmononuclearcellsthroughtheactivationofcaspase-1andIL-18secretioninmonocytes:W.R.Coward,etal.;J.Immunol.176,5284(2006)
Broad-spectrumcaspaseinhibitors:frommythtoreality?D.Chauvier,etal.;CellDeathDiffer.14,387(2007)
Acalpain-likeproteaseinhibitsautophagiccelldeath:D.T.Madden,etal.;Autophagy3,519(2007)
MalarialhemozoinisaNalp3inflammasomeactivatingdangersignal;C.Dostert,etal.;PLoSOne4,e6510(2009)