企业QQ
官方微信
| ProductDetails | |
|---|---|
| ProductType | Protein |
| Properties | |
| Source/Host | E.coli |
| Sequence | TwohumanubiquitinL73Pmutants(AccessionNr.P0CG47)covalentlylinkedthroughanisopeptidebondatK48residueofoneubiquitinmoleculeandtheC-terminalglycineresidueofanotherubiquitinmolecule,andcoupledtoagarose. |
| Crossreactivity | Human |
| Label/Conjugates | Agarose |
| Formulation | Liquid.Resinsuppliedasa50%slurryin20%ethanol. |
| OtherProductData | Use:Linkage-specific,non-hydrolyzabledi-ubiquitinisresistanttotheactivityofdeubiquitinatingenzymes(DUBs)thatcleavetheisopeptidelinkagebetweenadjacentubiquitinmolecules.Maybeusefulforinvestigatingubiquitin-bindingproteinsandexploringtheroleofunanchored(freeC-terminus)ubiquitinchainsinsignalingpathways.Usefulfortheenrichmentofknownubiquitinchain-interactingproteinsaswellasthediscoveryofnovelubiquitinchain-interactingproteins.Werecommendequilibratingtheresinbywashingwith10volumesofyourdesiredaqueousbuffer. Polyubiquitin-agarosecanbere-usedifproperlymaintained.Afteruse,cleanresinwithawashcycleof5volumes100mMHEPESpH8.0,500mMNaClfollowedby5volumes100mMNaOAcpH4.5,500mMNaCl.Repeattwice,thenrinseresinwithalowsaltbuffer. |
| Declaration | ManufacturedbyBostonBiochem |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | +4°C |
| HandlingAdvice | Donotfreeze. |
| Use/Stability | Stableforatleast3monthsafterreceiptwhenstoredat+4°C. |
| Documents | |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet |  DownloadPDF |