Transformedfromhuamnlungcancercellline,A549 bylentiviralsystemwithfireflyluciferase geneII and GFP.The Luciferaseand GFPwasco-expressedunderCMVpromoterasseperatedprotein(notasfusion). ThePuromycinMarkerwasexpressedunderRsvpromoter.Thecellconstitutivelyco-expressesbothfireflyLuciferaseandGFP.Thefollowingexpresscassettewasintegratedintocellline’sgenome.

EachcelldemonstratesstrongGFPfluorescentsignalandluciferaseassaysignal(Note:thisispooledstableellpopulation,notoriginated fromasinglecellcolony.Thusyouwillobserve theunevenGFPsignalintensityamountcells).
Pleaseseedetails in ProductManual.
Soldat: 1vialx(2 x106cells)/vial
Cat# SC043-LG