Single-colorFluorescenceReferenceStandardsarelabeledwithspecificFluorochromestoexhibitthesamespectralcharacteristicsaslabeledcells.Theirsizeapproximatesthatofhumanlymphocytes(~7-9µmindiameter)andareavailableinawidevareityoffluorescentdyes.Fluoresceinisalsoavailableasa2µmbead.ThesestandardsmaybeusedtoQCaspecificpathoftheopticalsystem(laser/filter/PMT),tooptimizefilterandmirrorsetsforfluorophores,andtoestablishatest-specificTargetChannelValueforinstrumentset-up.FormoreFlowCytometryResources,visittheBangsFlowBlog.
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