BioMag®anti-HumanCDIgG-coatedparticlesareproducedbycovalentlyattachingmurinemonoclonalantibodiestoBioMag®particles.Theantibodieswerepreparedagainsthumanleukocytedifferentiationantigens.EachproductissuppliedinphosphatebufferedsalinewithEDTAandsodiumazideaddedasstabilizers,andshouldbewashedinsterilemediapriortousetoremovethepreservative.
TheCD4antigenisexpressedonasubsetofperipheralbloodlymphocytes,theThelperlymphocytes.Itisalsopresentonmostthymocytesandisfrequentlyco-expressedwithCD8.CD4isalsoexpressedonallmonocytes,butatalowerdensitythanonCD4+Tlymphocytes.