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AntibodiesOnline/MagSIGNALSTA 300 beads/ABIN1721154/10 mL188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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AntibodiesOnline/MagSIGNALSTA 300 beads/ABIN1721154/10 mL
Antigen
Streptavidin
Application
Immunoassay(IA),Separation(Sep)
Options
Bulkdiscount
Supplier
SupplierProductNo.
PurposeSuperparamagneticparticlesof300nmwithhighqualitystreptavidincovalentlyattachedtothebeadsurface.Intendedforusewithbiotinylatedantibodiesasadetectionlabelinmagneticimmunoassays,orassolidsupportphaseinimmunoassayswithotherreadouttechniques.
CharacteristicsBindingCapacity:800-1600pmolbiotin/mgbeads
ComponentsMagneticbeadswithstreptavidinsurface
MaterialnotincludedMagnetforseparationofbeads
Vortexandshaker
ImmobilizationBuffer
WashingBuffer
ResuspensionBuffer
ProductDetails:BeadLigandStreptavidin
ProductDetails:BeadMatrixSuperparamagneticSilicaparticles
ProductDetails:BeadSizeBeadsize:300nm
ApplicationNotesMagSIGNALbeadsconsistofmanyferromagneticgrainswithrandomorientedmagneticmoments,encapsulatedinanon-magneticsilicashell.Thesizeoftheferromagneticgrainsis10-15nm.Duetothesmallsizeandrandomorientationofthemagneticmoments,thebeadsbehavesuperparamagneticallyandthemagneticmomentwithoutamagneticfieldis0(demonstratedinfigure1below).
Comment

Magneticimmunoassays(MIA)usemagneticbeadsaslabelsinsteadofconventionalenzymes(ELISA),fluorophoresorluminescentmolecules.Theseassaysinvolvethespecificbindingofanantibodywithaconjugatedmagneticlabeltoitsantigen.Thepresenceofmagneticbeadsisthendetectedbyamagneticreader(magnetometer)whichmeasuresthemagneticfieldchangeinducedbythebeads.Thesignalmeasuredbythemagnetometerisproportionaltotheanalyte(virus,toxin,bacteria,cardiacMarker,etc.)quantityintheinitialsample.
Magneticbeadsarenotaffectedbyreagentchemistryorphotobleachingandarethereforestableovertime.Becausemeasurementsarebasedonnonlinearmagneticsignals,thecontributionoflinearmagneticsignalsfrommaterialssuchassamplematrixandconsumablesareeliminated.Thetechnologymakesmagneticimmunoassayspossibleinavarietyofformatssuchasconventionallateralflowtest(replacinggoldlabelswithmagneticlabels),microfluidicapplicationsandbiochips.

ProtocolMagSIGNALaresuperparamagneticbeadswithameandiameterof300nm,whichcanbeusedasadetectionlabelinmagneticimmunoassays,orassolidsupportphaseinimmunoassayswithotherreadouttechniques.

MagSIGNAL-STA300iscoatedwithstreptavidinandisintendedforusewithbiotinylatedantibodies.BindingofmoleculesontoMagSIGNAL-STA300isbasedonthestrongnon-covalentinteractionbetweenstreptavidinandbiotin(Ka=1015M-1).Biotiniseasilyconjugatedtovariousmoleculesandinexpensivebiotinylatedproductsaresoldbymanycompanies.MagSIGNAL-STAbeadsarecoatedwithrecombinantstreptavidin(53kDa)withshortenedN-andC-terminusforimprovedsolubilityandaccessibilityofthesites.Albuminbindingsitesareeliminatedforoptimalspecificity.

Magneticcontent:90%
Density:2.5g/cm3
ReagentPreparation

ImmobilizationBuffer:0.01MPhosphateBufferedSaline(PBS)pH7.4
WashingBuffer:PBSpH7.4with0.05%Tween20
ResuspensionBuffer:PBSpH7.4with0.05%Tween20,0.05%

AssayProcedure

Immobilizationprotocol
Thefollowingprotocoldescribestheimmobilizationofbiotinylatedmoleculeson1mL(10mg)beads.Itcanbescaledupbyadjustingvolumesofrequiredreagents.
1.ResuspendMagSIGNAL-STA300byvortexing.Transfer1mLfromthestoragebottletoareactioncontainer.
2.Preparethebeads:Placethereactioncontainerinamagneticseparatorandwaituntiltheseparationiscompleted.Discardthebufferandresuspendin1mLImmobilizationbuffer.Repeatthissteponcemore.
3.AddbiotinylatedmoleculestothebeadsandaddImmobilizationBuffertoafinalvolumeof1mL.Incubatethebeadsonashakerfor30minutesatroomtemperature.
4.Placethereactioncontainerinamagneticseparator,waituntiltheseparationiscompleted,anddiscardthesupernatant.
5.Washthebeads3xwith1mLWashingBuffer.
6.Finallyresuspendthebeadsin1mLResuspensionBufferandstoreat2-8°C.

RestrictionsForResearchUseonly
FormatLiquid
Concentration10mg/mL
BufferPBS(pH7.4),0.05%Tween20,0.05%Sodiumazide
PreservativeSodiumazide
PrecautionofUseThisproductcontainssodiumazide:aPOISONOUSANDHAZARDOUSSUBSTANCEwhichshouldbehandledbytrainedstaffonly.
HandlingAdviceStoreinwellclosedvialandinuprightpositiontopreventdrying,thismayresultinadecreaseofactivity.
Donotfreezetheproduct!
Vortexwellbeforeuse.Washthebeadstoremovepreservativesthatcouldinterferewithyourappl
Storage4°C
ExpiryDate12months
SupplierImages
image for MagSIGNAL-STA 300 beads (ABIN1721154)MagSIGNAL-STA300beads
image for MagSIGNAL-STA 300 beads (ABIN1721154)MagnetizationcurveofMagSIGNAL(sampleamount:15,4mg,normalizedmomentat700Oe...

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