Abstract:
Thispaperdescribestheutilizationofgiantunilamellarvesicles(GUVs)asaplatformforhandlingchemicalandbiochemicalreagents.GUVswithdiametersof5to10μmandcontainingchemical/biochemicalreagentstogetherwithinertpolymerswerefusedwithelectricpulses(electrofusion).Afterreagentmixing,thefusedGUVsspontaneouslydeformedtoabuddingshape,separatingthemixedsolutionintosub-volumes.WeutilizedamicrofluidicchannelandopticaltweezerstoselectGUVsofinterest,bringthemintocontact,andfusethemtogethertomixandaliquotthereactionproduct.Wealsoshowthat,byloweringtheambienttemperatureclosetothephasetransitiontemperatureTmofthelipidused,daughterGUVscompletelydetached(fission).Thisprocessperformsalltheliquid-handingfeaturesusedinbench-topbiochemistryusingtheGUV,whichcouldbeadvantageousforthemembrane-relatedbiochemicalassays.
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