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| Synonyms | MonoclonalM13+fd+F1FilamentousPhagesantibody,Anti-M13+fd+F1FilamentousPhagesantibody,M+fd+F1FilamentousPhages13,M13+fd+F1FilamentousPhages,M+fd+F1FilamentousPhages-13antibody,M13/fd/F1FilamentousPhagesantibody,M+fd+F1FilamentousPhages-13,M+fd+F1FilamentousPhages13antibody |
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| Specificity | M13phage |
| CrossReactivity | ThisantibodyrecognizesanepitopeonpVIII(phagecoatprotein)coveringtheN-Terminalregionoftheg8pAEGDDPAKAAFDSLQASAT |
| Applications | ELISA,IF,PhageDisplay,WB |
| Immunogen | M13/fd/F1filamentousphagesantibodywasraisedinmouseusingfdphagesfromE.ColiF+strainastheimmunogen. |
| Host | Mouse |
|---|---|
| Clone | B62-FE2 |
| Isotype | IgG2b |
| MethodofPurification | ProteinAaffinitychromatography |
| Form&Buffer | Reconstitutein0.1mldistilledwater.(finalsolutioncontains10mMTris-HClbuffer,pH8.0,300mMNaCl). |
| Storage | Storeat4degCuntilreconstitution.Followingreconstitutionaliquotandfreezeat-20degCforlongtermstorage.Avoidrepeatedfreeze-thawcycles. |
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| UsageRecommendations | ELISA:1:50,000,PhageDisplaydetectionlimit=10^7phageparticles |
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| BiologicalSignificance | M13isafilamentousbacteriophagecomposedofcircularsinglestrandedDNA(ssDNA)whichis6407nucleotideslongencapsulatedinapproximately2700copiesofthemajorcoatproteinP8,andcappedwith5copiesoftwodifferentminorcoatproteins(P9,P6,P3)ontheends.TheminorcoatproteinP3attachestothereceptoratthetipoftheFpilusofthehostEscherichiacoli.Infectionwithfilamentousphagesisnotlethal,howevertheinfectioncausesturbidplaquesinE.coli. |
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