美国MTIglobalstem总代理GST2161/现货

　　产品介绍：

产品名称：DNA-In®CRISPRTransfectionReagent

产品描述：

NEWDNA-In®CRISPRTransfectionReagent

SpecificallyformulatedforCRISPR/Cas9transfectionreagent

DNA-In®CRISPRtransfectionreagentisoptimizedforlargeplasmiddeliveryofCRISPR/Cas9vectorsintoarangeofcelltypes.Thisreagentisespeciallywell-suitedforhard-to-transfectprimarycells.Below,aCas9-GFPplasmidwasdeliveredintoprimaryfibroblasts,musclescells,keratinocytesandothersprimarycellsandcommonlyusedcelltypeswithhighefficiencyandlowtoxicity.

Figure1.DNA-In®CRISPRdeliversCas9-GFPexpressionvectorwithmaximumefficiency-Humanprimaryfibroblasts,humanprimarykeratinocytes,primaryhumanendothelialcells(HuVEC),humanskeletalmusclecells,HeLacellsandC2C12mousemyoblastswereplatedin24-wellplatesincompletemediumwithoutantibioticsattheoptimalplatingdensityforeachcelltypetoyield50-70%confluencyatthetimeoftransfection.CellsweretransfectedwiththepSpCas9(BB)-2A-GFPpasmid(9.3kb)(source:Addgene),usingDNA-In®CRISPRreagentandincubatedovernightat37°C.Cellswerethenfixedandstainedwithanti-GFPandanti-Cas9antibodiestobetterobservethetransfectionefficiency.

LearnaboutHighEfficiencyCRISPR-Cas9DeliveryinStemCells

HighlyefficientCas9deliveryinprimarycells

Humandermalprimaryfibroblasts(above)wereplatedtogive~70%platingdensityonthedayoftransfection.CellsweretransfectedwithCas9-GFP24hoursafterplating.Cellswerelabelledwithanti-GFP(green)toobservelowexpressionofCas9-GFPvectorandHoechst3342(blue)toviewnuclei.DNA-In®CRISPRtransfectedcells(leftimage)showsignificantlyhigherCas9transfectionefficiencyvscellstransfectedwithcompetitorreagents(L2K,L3K).

Humanprimarykeratinocytes(above)wereplatedtogivea50-70%platingdensityonthedayoftransfection.CellsweretransfectedwithCas9-GFP24hoursafterplating.Cellswerelabelledwithanti-GFP(green)toobservelowexpressionofCas9-GFPvectorandHoechst3342(blue)toviewnuclei.DNA-In®CRISPRtransfectedcells(leftimage)showsignificantlyhigherCas9transfectionefficiencyvscellstransfectedwithcompetitorreagents(L2K,L3K).

C2C12mousemyoblasts(above)wereplatedtogivea50-70%platingdensityonthedayoftransfection.CellsweretransfectedwithCas9-GFP24hoursafterplating.Cellswerelabelledwithanti-GFP(green)toobservelowexpressionofCas9-GFPvectorandHoechst3342(blue)toviewnuclei.DNA-In®CRISPRtransfectedcells(leftimage)showsignificantlyhigherCas9transfectionefficiencyvscellstransfectedwithcompetitorreagents(L2K,L3K).

Humanskeletalmusclecells(above)wereplatedtogivea50-70%platingdensityonthedayoftransfection.CellsweretransfectedwithCas9-GFP24hoursafterplating.Cellswerelabelledwithanti-GFP(green)toobservelowexpressionofCas9-GFPvectorandHoechst3342(blue)toviewnuclei.DNA-In®CRISPRtransfectedcells(leftimage)showsignificantlyhigherCas9transfectionefficiencyvscellstransfectedwithcompetitorreagents(L2K,L3K).

　　公司介绍

mti-globalstem，是生命科学领域前沿工具和试剂的供应商。mti-globalstem提供优势产品在转染试剂、神经科学、干细胞生物学。他们填补了原代和ipsc获得神经细胞、干细胞和饲养层细胞的空白，优化了生长培养基和补充物质，提供了一个贵族试剂的创新平台——最大效率的递送核酸到干细胞和神经细胞。

MTI-GlobalStem公司成立于2006年，最初名为GlobalStem，在2010年被MolecularTransfer公司(MTI)收购，总部位于美国马里兰州盖瑟斯堡，致力于开发和推广细胞转染、神经生物学以及干细胞研究所用的工具和试剂。此外，这家公司还提供干细胞、分化神经元和大规模的细胞培养制造服务。

蚂蚁淘生物是美国MTI-globalstem公司,GST-2161-DNA-In®CRISPRTransfectionReagent/现货/代理商