DNA-In®TransfectionReagent

AUniversalTransfectionReagentforDNADelivery

DNA-In®isthenewestgenerationtransfectionproductdevelopedbytheleadmembersofthescientificteamthatinventedtheLipofectamine®andLipofectamine®2000.DNA-In®isanovelcationictransfectionreagentwhichbalanceshightransfectionandexpressionlevelswithlowtoxicity.DNA-In®worksonaverybroadrangeofadherentcellsincludingmostpassagedcelllinesandmanyprimarycells.

• Productsheet

• Features

• Fast,simple,easy-to-followprotocol
  

• One-stepprocess:simplyaddDNA-In®ReagenttodilutedDNA,incubate10minutes,andaddtopre-platedcells
  

• Minimaltoxicity-noneedtoreplacemediumaftertransfection
  

DNA-In®TransfectionReagent"Quick-StartProtocol"

SuperiorTransfectionEfficiency

Duringitsdevelopmentandvalidationprocess,DNA-In®Reagentunderwentextensivetestinginmanyprimaryandculturedcells.Inahead-to-headcomparisonstudy,DNA-In®consistentlyoutperformedtheLipofectamine®2000transfectionreagent.

Cellsweretransfectedwithaplasmid,containingEmeraldGFPbehindanEF1αpromoter,witheitherDNA-In®orLipofectamine®2000,atseveraldifferentlipid:DNAratiosandexamined~44hourspost-transfection.Representativeimagesofthebestconditionsforeachtransfectionreagentareshown.

LowCellToxicity

DNA-In®hasasignificantlylowercelltoxicitycomparedtotraditionaltransfectionreagentssuchasLipofectamine®andLipofectamine®2000.Inthemanycelllinestested,DNA-In®showsabroadrangeofeffectivedosagewhilemaintainingminimalcelltoxicity.Thislowcelltoxicityallowsalargerdosageandtimewindowforoptimizedtransfectionresults.

Thefollowingchartsshowside-by-sidecomparisonsofDNA-In®andLipofectamine®2000(LF2K)incommonlyusedcelllinesandprimarycells:

In293cells,DNA-In®maintainshighcellviabilitythroughoutthetesteddosagerange,whileLF2Khasasignificantcelltoxicityissuewhenusedindosagesover3µl.

InA549cells,DNA-In®hasasignificantlyhighertransfectionefficiencyandalowercelltoxicitywhileLF2Kperformspoorlyinbothparameters.

Inhumanfibroblastcells,DNA-In®hasasignificantlyhighertransfectionefficiencyandalowercelltoxicitywhileLF2Kperformspoorlyinbothparameters.

InHeLacells,DNA-InhasasignificantlyhighertransfectionefficiencyandalowercelltoxicitywhileLF2Kperformspoorlyinbothparameters.

InHepG2cells,DNA-InhasasignificantlyhighertransfectionefficiencyandalowercelltoxicitywhileLF2Kperformspoorlyinbothparameters.

InMCF7cells,DNA-InhasasignificantlyhighertransfectionefficiencyandalowercelltoxicitywhileLF2Ktransfectionefficiencyislowerandistoxicwhenusedindosageshigherthan2µl.