mRNAIn® Transfection Reagent

AUniversalTransfectionReagentforHighEfficiencymRNADelivery

mRNA-In®TransfectionReagentwasdevelopedbymembersofthescientificteamthatinventedLipofectamine®andLipofectamine®2000andspecificallyformulatedforinvitromRNAdelivery.MaximumRNAdeliveryisachievedwithmRNA-In®usingloweramountsofmRNAthanotherreagents,reducingexperimentalcostsandsignificantlyloweringtoxicity.Acrossawiderangeofcelltypes,mRNA-In™hasshowntoproduceexceptionallyhightransfectionefficiencywhilemaintainingoptimalcellhealthandviability.

Features

• RequireslowamountsofRNA-reducecostandpotentialoff-targettoxicityeffectswithlowamountsofRNArequirements.
  

• Transfectsavarietyofcelllineswithaslittleas2pg/cellofmRNA

• Highexpression,lowtoxicity-achievehighmRNAefficiencywhilemaintainingoptimalcellhealthforuncompromisedexperimentresults.
  

• Robust,versatileperformance-produceefficienttransfectionacrossawiderangeofcelltype,includingstemcellsandprimarycells.

Applications

mRNA-In®TransfectionReagentisidealformRNAdeliveryforvariousapplications,suchasproteinexpressionandiPSC-reprogramming.DatabelowalsoshowsmRNA-In®significantlyoutperformscompetitorreagents,typicallyachieving90%orgreatertransfectionefficiencyacrossawiderangeofcelltypes.

• MakingtheOptimalMessengerRNAforGeneTherapyApplications.pdf[TriLink]

• AReproducible,EfficientandUser-friendlyRNAReprogrammingSystem.pdf[ESIBio]

DeliversMaximumTransfectionEfficiencyusingLowAmountsofmRNA

DatabelowshowcommonlyusedcelltypestransfectedwithmRNA-In®TransfectionReagentusingvariousamountsofmRNA.Cellswereplatedin24-wellplatestogive60-70%confluencethedayoftransfection.GFP-mRNAcontaining5-methlycytosineandpsuedouridinewerecomplexedwithvariousamountsofmRNA-In®inatotalof50µlofOptiMEM®.ForeachexperimentmRNA/mRNA-In™complexeswereaddedtocells,mixed,andincubatedat37ºCin5%CO2.Cellswereobserved24hoursfollowingtransfection.

HigherExpression,SignificantlyOutperformstheLeadingCompetitors

Below,SH-SY5Y(neuroblastomacells),HDF(humandermalfibroblasts),HUVEC(humanumbilicalveinendothelialcells),HeLaandadipose-derivedMesenchymalStemCells(MSCs)weretransfectedwithmRNA-In™orMessengerMAX™(LifeTechnologies)usinglowamountsofmRNA(100ng).Cellswereplatedin24-wellplatestogive60-70%confluencethedayoftransfection.GFP-mRNAcontaining5-methlycytosineandpsuedouridinewerecomplexedwiththevariousamountsofmRNA-In®(seechartlegends).RNA/reagentcomplexeswereaddedtothecells,mixed,andincubatedat37ºCin5%CO2overnight.Cellswereanalyzedbyafluorescenceplatereaderandmicroscopy24hourspost-transfection.Errorbarsrepresentthestandarddeviationoftriplicatewells.ThedatabelowshowsmRNA-In™providessignificantlyhighertransfectionefficiencythanthecompetitorreagent.