EGFR (C775S T790M L858R) Kinase Enzyme System

我们使用cookies和类似技术来维护我们的网站运转，进行数据分析，改进我们的网站，并向您展示个性化的内容和广告。其中有些cookies在网站运行中是必不可少的。对于其他cookies，除非你允许否则我们不会使用。想要了解更多关于cookies和如何管理cookies，请阅读我们的Cookie政策。 拒绝所有 管理Cookies 同意所有 A password reset email has been sent to the primary email address associated with your account. There was an issue with the password reset process. Please try again or contact Customer Service. Contact Customer Service You have successfully reset your password. Log in with Your New Password There was an issue resetting your password. Please try again or contact Customer Service. Contact Customer Service You have not verified your email address. A verified email address is required to access the full functionality of your Promega.com account. Resend Verification Email There was an issue logging into your account. Please try again or contact Customer Service. Contact Customer Service A verification email has been sent to the primary email address associated with your account. There was an issue sending the verification email. Please try again or contact Customer Service. Contact Customer Service Congratulations! You\'ve created a Promega.com account. Check your inbox to complete email verification. Note: You will not be able to access your account until your email is verified. There was an issue creating your account. Please try again or contact Customer Service. Contact Customer Service There was an error processing your request. Please check your network settings and try again. There was an issue verifying your email address. Please try again or contact Customer Service. Contact Customer Service The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The EGFR (C775S T790M L858R)Kinase Enzyme System contains: EGFR (C775S T790M L858R) Kinase, 10 g (Human, recombinant; amino acids 695 end). MW: ~89kDa Poly (4:1 Glu, Tyr)Substrate (1mg/ml) 5X Reaction Buffer, 2.5M MnCl2, 0.1M DTT Recombinant human EGFR (C775S T790M L858R) (amino acids 695 end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility and cell survival. Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms. Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation. Application Note NCBI Database Entry The EGFR (C775S T790M L858R)Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request. Use with ADP-Glo™ Kinase Assay The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. European Pat. No. 1131441 and Japanese Pat. No. 4520084. U.S. Pat. No. 8,183,007 and other patents and patents pending. U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents. U.S. Pat. No. 7,700,310 and other patents and patents pending. U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767and other patents and patents pending. Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents. 我们使用这些cookies以确保我们的网站安全及正常运转；它们是我们服务功能中所必需的，且不能在我们的系统中关闭。它们通常只在您有操作需求时才会响应，例如登录账号、使用购物车或填表时。关于这些cookies，您可以设置浏览器阻止或提醒，但如果没有它们的话我们将无法提供部分服务。与我们使用的其他cookies一样，绝对必需的cookies既可能是第一方cookies也可能是第三方cookies。 有关偏好的cookies 我们使用这些cookies来记住您的设置和偏好。譬如，我们可能会使用这些cookies来记住您的语言偏好。 Allow 有关偏好的cookies 有关性能和统计的cookies 我们使用这些cookies来收集您与我们的互动信息，以此作为评判和改进我们服务的依据。譬如，我们可能会使用这些cookies来判断您是否在某一特定页面有过互动。 Allow 有关性能和统计的cookies 有关营销的cookies 我们和我们的广告合作商使用这些cookies投放跟您关联且有意义的广告，并且可以及时追踪我们广告投放活动的效果，无论是在我们的网站还是在其他网站或社交媒体上。 Allow 有关营销的cookies