JPT/SpikeMix™ Plant Organelle Marker (Arabidopsis thaliana) heavy/SPTORGPOOLL/

SpikeMix™ Plant Organelle Marker (Arabidopsis thaliana) - heavy

Product Code: SPT-ORG-POOL-L

Pool of 67 stable isotope-labeled organelle marker peptides from Arabidopsis thaliana organelles. It covers 14 major subcellular locations: Cytosol, Cytosol (cytosolic ribosomes), ER, Extracellular, Golgi, Mitochondria, Nucleus, Peroxisome, Plastid (LHC), Plastid (non-LHC), PM, Vacuole, Nucleus, Mitochondria. Peptide mixtures for protein quantification by mass spectrometry based proteomics (e.g. MRM).
Amount: Pool of 67 proteotypic stable isotope labeled peptides (50 -150 pmol/peptide)
Delivery Format: Polypropylene vial
Application(s):MRM assays, Proteomics, SRM assays, Mass spectrometry, Organelle isolation
Indication(s)/Topic(s): Control, Organelle abundance
Delivery Time: 2-5 days

JPTs SpikeMix™
JPT produces specific mixtures of stable isotope-labeled peptides for the detection of relevant proteins by mass-spectrometry based proteomics. The mixed peptides are available as stable isotope labeled peptides (using heavy Arg U-13C6, U-15N4 or Lys U-13C6, U-15N2).

Benefits of JPTs SpikeMix™
- Quantification of multiple proteins from a single sample
- Economic premixed heavy peptide sets available for different species
- Multiplexed analysis of disease status and therapeutic success

SpikeMix™ Plant Organelle Marker (Arabidopsis thaliana) - heavy

Selected References
"Quantitative Proteomics of Bronchoalveolar Lavage Fluid in Idiopathic Pulmonary Fibrosis"
Foster et al., J of Proteome Research (2015) - PMID: 25749006
"Biomarker Development for Intraductal Papillary Mucinous Neoplasms Using Multiple Reaction Monitoring Mass Spectrometry"
Kim et al., J. Proteome Res (2015) - PMID: 26561977
"Development and Validation of a Novel Plasma Protein Signature for Breast Cancer Diagnosis by Using Multiple Reaction Monitoring-based Mass Spectrometry"
Lee et al., Anticancer Research (2015) - PMID: 26504062
"Non-invasive Prognostic Protein Biomarker Signatures Associated with Colorectal Cancer"
Surinova et al., EMBO Mol Med. (2015) - PMID: 26253080

Application Notes
"Development of a Multiplexed Targeted SRM Assay for NCI’s Top Tumor Associated Antigens"
Soderblom et al., Application Note (2015) Full text
"Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides"
Bindel et al., Application Note (2015) Full text
"Development and Characterization of SpikeMix™ ABRF (cross-Species Standard) Consisting of 1,000 Stable Isotope Labeled Peptides"
Colangelo, Application Note (2014) Full text

Testimonials
"As Director of the Protein Profiling at Yale Keck Biotechnology Resource Facility, I coordinated a collaboration between the Association of Biomolecular Resource Facilities (ABRF) standard proteomic research group (sPRG) and JPT Peptide Technologies to develop the ABRF cross-species SpikeMix™ peptide pool. The joint development turned out to be an extraordinary effective endeavor and the resulting product was qualified in more than 52 proteomics labs around the globe. I was impressed by JPTs scientific and technological capabilities as well as their enthusiasm to drive the project forward."
Christopher M. Colangelo, Director of Protein Profiling at Keck Biotechnology Resource Facility, Yale University, USA

"Our research at Stanford University seeks to discover unconventional translation products, and proteins made by unsequenced organisms; to characterize a wide array of protein post-translational modifications; and to perform peptide antigen binding assays. On all of these fronts, we are in need of high quality peptide standards for assay development and protein quantification. The successful collaboration with the team at JPT Peptide Technologies and their wide range of products has been a critical factor for advancing my groups research."
Joshua E. Elias, Ph.D., Assistant Professor, Chemical & Systems Biology, Stanford University School of Medicine, Clark Center, Stanford, CA