JPT/PepMix™ Arabidopsis (WRKY47)/PMWRK/

PepMix™ Arabidopsis (WRKY47)

Product Code: PM-WRK

Pool of 120 peptides derived from a peptide scan (15mers with 11 aa overlap) through Probable WRKY transcription factor 47 (Swiss-Prot ID: Q9ZSI7) of Arabidopsis thaliana (Mouse-ear cress).
Amount: 1 vial containing 15 nmol (appr. 25µg) per peptide
Purity: >70% (HPLC-MS)
Delivery Format: Freeze dried in amber type glass vial
Application(s): T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response
Indication(s)/Topic(s): Cancer
Delivery Time: 2-5 days

PepMixes™ Peptide Pools
The use of complete protein-spanning mixtures of overlapping peptides (PepMix™) is extremely efficient for immunostimulation of T-lymphocytes and diagnostic applications. In order to establish reliable and validated T-cell assays such as ELISPOT, appropriate positive and negative controls are essential. Selection of such controls helps to confirm proper functionality of the assay as well as viability of the cells used. In addition, high quality controls are most important to establish standardized assay protocols in clinical setups. Compared to commonly used controls like phytohemagglutinin (PHA) and concanavalin A (ConA) that stimulate T-cells as well as other cell types or full length antigens, synthetic peptide pools offer the advantage of a high batch-to-batch reproducibility, applicability of reliable chemical and biochemical QC/QA measures and shelf stability when stored as freeze dried aliquots.

Benefits of PepMix™
- Equivalent or better stimulation of CD4⁺ and CD8⁺ T-cell responses compared to whole protein antigens
- Simultaneous detection of CD4⁺ and CD8⁺ responses in a single sample
- Improved responses in stored blood and PBM cells compared to whole protein antigens
- More reliable as control than phytohemagglutinin (PHA) and concanavalin A (ConA)
- High-batch-to-batch reproducibility
- Reliable chemical and biochemical quality control and quality assurance for peptide synthesis and peptide pool generation
- Prolonged shelf stability when stored freeze dried

PepMix™ Arabidopsis (WRKY47)

Selected References
"Dysfunction of PSA-specific CD8+ T Cells in Prostate Cancer Patients Correlates with CD38 and Tim-3 Expression"
Japp et al., Cancer Immunol Immunother. (2015) - PMID: 26289091
"An HPV-E6/E7 Immunotherapy Plus PD-1 Checkpoint Inhibition Results in Tumor Regression and Reduction in PD-L1 Expression"
Rice et al., Cancer Gene Therapy (2015) - PMID: 26337747
"Survivin-specific CD4+ T Cells are Decreased in Patients with Survivin-positive Myeloma"
Locke et al., J Immunother Cancer (2015) - PMID: 25992291
"A Phase I Trial Combining Decitabine/dendritic Cell Vaccine Targeting MAGE-A1, MAGE-A3 and NY-ESO-1 for Children with Relapsed or Therapy-Refractory Neuroblastoma and Sarcoma"
Krishnadas et al., Cancer Immunol Immunother. (2015) - PMID: 26105625
"Guidelines For the Automated Evaluation of Elispot Assays"
Janetzki et al., Nature Protocols (2015) - PMID: 26110715
"Presence of Circulating Her-2-reactive CD8 + T-Cells is Associated with Lower Frequencies of Myeloid-derived Suppressor Cells and Regulatory T Cells, and Better Survival in Elderly Breast Cancer Patients"
Kini Bailur et al., Breast Cancer Research (2015) - PMID: n.a.
"STAT3 Signaling Contributes to the High Effector Activities of Interleukin-15-derived Dendritic Cells"
Okada et al., Immunol Cell Biol. (2015) - PMID: 25582338

Application Notes
"Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy"
Lam et al. (2015) Full text
"Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation"
Gary et al. (2015) Full text

Testimonials
"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our "go-to" company for purchasing peptides."
Dr. Laura J Pallett, Infection and Immunity, University College London, UK